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PMID: 673837 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNA associated with hyperacetylated histone is preferentially digested by DNase I.

Nucleic acids research ·Vol. 5 ·No. 6 ·1978-06-00 ·Pages 1863-76

Sealy L, Chalkley R

Abstract

Butyrate-treated cells give rise to massive hyperacetylation of histones and have been used to test the idea that regions of DNA in association with hyperacetylated histones are preferentially solubilized upon digestion with DNase I. Such hyperacetylated histones can be derived from both pre-existing histones or from histone newly synthesized in the presence of butyrate which leads to extreme modification. The DNA in association with both types of hypermodified histone is equally and selectively digested.

MeSH Terms
Acetylation Butyrates/pharmacology Cell Line DNA Deoxyribonucleases Histones Kinetics Protein Binding
Chemicals
Butyrates Histones DNA Deoxyribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sealy L
Chalkley R
References (35)
35 references, click to expand
  1. ACETYLATION AND METHYLATION OF HISTONES AND THEIR POSSIBLE ROLE IN THE REGULATION OF RNA SYNTHESIS.
    Proc Natl Acad Sci U S A. 1964 May;51:786-94 PMID: 14172992
  2. Activation of chromatin by acetylation of histone side chains.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3937-41 PMID: 1069278
  3. Analysis and reconstruction of Xenopus ribosomal chromatin nucleosomes.
    Eur J Biochem. 1977 May 16;75(2):545-60 PMID: 885142
  4. Acetylation and phosphorylation of Drosophila histones. Distribution of acetate and phosphate groups in fractionated chromatin.
    Biochim Biophys Acta. 1977 Mar 2;475(1):168-75 PMID: 191076
  5. Chromatin.
    Nature. 1978 Jan 12;271(5641):115-22 PMID: 340956
  6. n-Butyrate causes histone modification in HeLa and Friend erythroleukaemia cells.
    Nature. 1977 Aug 4;268(5619):462-4 PMID: 268489
  7. An altered subunit configuration associated with the actively transcribed DNA of integrated adenovirus genes.
    Cell. 1977 Nov;12(3):783-94 PMID: 562719
  8. Selective degradation of integrated murine leukemia proviral DNA by deoxyribonucleases.
    Cell. 1977 Aug;11(4):933-40 PMID: 560916
  9. Ultrastructural patterns of RNA synthesis during early embryogenesis of Drosophila melanogaster.
    Cell. 1976 Jun;8(2):305-19 PMID: 822943
  10. Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3966-70 PMID: 1069279
  11. Comparative organization of active transcription units in Oncopeltus fasciatus.
    Cell. 1976 Sep;9(1):131-46 PMID: 975237
  12. The structure of the globin genes in chromatin.
    Biochemistry. 1975 Jun 3;14(11):2489-95 PMID: 1095055
  13. Analysis of DNA of isolated chromatin subunits.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3978-82 PMID: 1060078
  14. Regulation of tyrosine hydroxylase activity in cultured mouse neuroblastoma cells: elevation induced by analogs of adenosine 3':5'-cyclic monophosphate.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2241-5 PMID: 4403308
  15. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  16. A study of adenosine 3':5'-cyclic monophosphate, sodium butyrate and cortisol as inducers of HeLa alkaline phosphatase.
    Arch Biochem Biophys. 1974 Oct;164(2):619-23 PMID: 4376666
  17. Lysine-rich histone phosphorylation. A positive correlation with cell replication.
    Biochemistry. 1972 Mar 14;11(6):1094-8 PMID: 4335288
  18. Turnover of deoxyribonucleic acid, histones, and lysine-rich histone phosphate in hepatoma tissue culture cells.
    Biochemistry. 1972 Oct 10;11(21):3915-21 PMID: 4342897
  19. Phosphorylation of liver histone following the administration of glucagon and insulin.
    Proc Natl Acad Sci U S A. 1969 Dec;64(4):1276-83 PMID: 4318347
  20. A comparative study of histone acetylation, histone deacetylation, and ribonucleic acid synthesis in avian reticulocytes and erythrocytes.
    Biochemistry. 1973 Feb 27;12(5):783-91 PMID: 4568767
  21. Effect of sodium butyrate on mammalian cells in culture: a review.
    In Vitro. 1976 Feb;12(2):125-32 PMID: 2548
  22. Phosphorylation of distinct regions of f1 histone. Relationship to the cell cycle.
    J Biol Chem. 1976 Jun 25;251(12):3685-92 PMID: 180006
  23. Phosphorylation of the lysine-rich histones throughout the cell cycle.
    Biochemistry. 1975 Jun 3;14(11):2504-11 PMID: 166660
  24. Studies on highly metabolically active acetylation and phosphorylation of histones.
    J Biol Chem. 1975 Jul 10;250(13):4856-63 PMID: 168194
  25. Cell cycle-specific changes in histone phosphorylation associated with cell proliferation and chromosome condensation.
    J Cell Biol. 1974 Feb;60(2):356-64 PMID: 4855902
  26. Induction of glycolipid biosynthesis by sodium butyrate in HeLa cells.
    Biochem Biophys Res Commun. 1974 Jul 10;59(1):292-9 PMID: 4858378
  27. Histones: structure and function.
    Annu Rev Biochem. 1971;40:279-314 PMID: 4941236
  28. Subunit structure of rDNA-containing chromatin.
    Biochemistry. 1976 Feb 24;15(4):750-5 PMID: 813764
  29. Modifications to histones immediately after synthesis.
    J Mol Biol. 1976 Jun 25;104(2):471-83 PMID: 950668
  30. Production of human chorionic gonadotropin in HeLa cell cultures.
    Nature. 1976 Feb 5;259(5542):416-7 PMID: 1250386
  31. Processing of newly synthesized histone molecules.
    Science. 1975 Oct 10;190(4210):117-28 PMID: 1166303
  32. Changing patterns of histone acetylation and RNA synthesis in regeneration of the liver.
    Proc Natl Acad Sci U S A. 1968 Apr;59(4):1337-44 PMID: 5240033
  33. RNA synthesis and histone acetylation during the course of gene activation in lymphocytes.
    Proc Natl Acad Sci U S A. 1966 Apr;55(4):805-12 PMID: 5219687
  34. Sites of in vivo acetylation in trout testis histone IV.
    J Biol Chem. 1971 May 25;246(10):3182-8 PMID: 5574393
  35. Chemical studies of histone acetylation. The distribution of epsilon-N-acetyllysine in calf thymus histones.
    J Biol Chem. 1968 Dec 25;243(24):6361-6 PMID: 5726891
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.6.1863
Published
1978-06-00
Pages
1863-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342130
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.6.1863 PMC Full Text View journal details
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