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PMID: 6351067 Published · ppublish English Comparative Study Journal Article

Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 19 ·1983-10-00 ·Pages 5852-6

Zimmerman SB, Pheiffer BH

Abstract

In the presence of high concentrations of any of several types of macromolecules, DNA ligase preparations from rat liver nuclei or from Escherichia coli actively catalyze the blunt-end ligation of DNA. This is in contrast to the lack of activity on such substrates by these enzymes under conventional assay conditions. In addition, the previously established activity of T4 DNA ligase on blunt-ended molecules is greatly increased in the presence of high concentrations of macromolecules. Because such crowded solutions may well be a more adequate model for intracellular conditions than assays in dilute solutions, we suggest that blunt-end ligation may be a widely occurring reaction in vivo.

MeSH Terms
Animals Cell Nucleus/enzymology DNA Ligases/metabolism Escherichia coli/enzymology Kinetics Liver/enzymology Polynucleotide Ligases/metabolism Rats Species Specificity Substrate Specificity T-Phages/enzymology Temperature
Chemicals
DNA Ligases Polynucleotide Ligases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Zimmerman S B
Pheiffer B H
References (35)
35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.19.5852
Published
1983-10-00
Pages
5852-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390173
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.19.5852 PMC Full Text View journal details
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