Home LiteratureArticle Details
PMID: 6272209 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Two new restriction endonucleases from Proteus vulgaris.

Nucleic acids research ·Vol. 9 ·No. 18 ·1981-09-25 ·Pages 4525-36

Gingeras TR, Greenough L, Schildkraut I, Roberts RJ

Abstract

Two novel sequence-specific endonucleases have been isolated from Proteus vulgaris, ATCC 13315. PvuI recognizes the sequence: 5' C G A T decrease C G 3' 3' G C increase T A G C 5' and PvuII recognizes the sequence: 5' C A G decrease C T G 3' 3' G T C increase G A C 5' and cleave as indicated by the arrow (decrease). PvuI is an isoschizomer of XorII, RshI, and XniI. No enzyme with the specificity of PvuII has been described previously.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes/isolation & purification,metabolism Deoxyribonucleases Pancreas/enzymology Proteus vulgaris/enzymology Substrate Specificity
Chemicals
Deoxyribonucleases DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gingeras T R
Greenough L
Schildkraut I
Roberts R J
References (14)
14 references, click to expand
  1. DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.
    Nucleic Acids Res. 1974 Mar;1(3):331-53 PMID: 10793670
  2. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.
    Eur J Biochem. 1969 Dec;11(2):395-9 PMID: 4902612
  3. A specific endonuclease from Arthrobacter luteus.
    J Mol Biol. 1976 Mar 25;102(1):157-65 PMID: 1271462
  4. The mapping and sequence determination of the single site in phiX174am3 replicative form DNA cleaved by restriction endonuclease Pst I.
    FEBS Lett. 1976 Jun 15;65(3):284-7 PMID: 955064
  5. Recognition sequence of specific endonuclease BamH.I from Bacillus amyloliquefaciens H.
    Nature. 1977 Jan 6;265(5589):82-4 PMID: 834250
  6. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  7. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  8. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  9. The cleavage site of the restriction endonuclease Ava II.
    Nucleic Acids Res. 1978 Jul;5(7):2313-9 PMID: 97636
  10. A computer assisted method for the determination of restriction enzyme recognifion sites.
    Nucleic Acids Res. 1978 Nov;5(11):4105-27 PMID: 724510
  11. Characterization of a site-specific restriction endonuclease from Rhodopseudomonas sphaeroides.
    J Bacteriol. 1979 May;138(2):505-9 PMID: 312287
  12. Two sequence-specific endonucleases from Xanthomonas oryzae. Characterization and unusual properties.
    Biochim Biophys Acta. 1980 Feb 29;606(2):371-85 PMID: 6243986
  13. Restriction and modification enzymes and their recognition sequences.
    Nucleic Acids Res. 1981 Jan 10;9(1):r75-96 PMID: 6259615
  14. Two sequence-specific endonucleases from Moraxella bovis.
    J Mol Biol. 1977 Jul;114(1):169-79 PMID: 909085
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.18.4525
Published
1981-09-25
Pages
4525-36
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327455
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.18.4525 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2