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PMID: 6259621 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Temperature dependence of the joining by T4 DNA ligase of termini produced by type II restriction endonucleases.

Nucleic acids research ·Vol. 9 ·No. 1 ·1981-01-10 ·Pages 85-93

Ferretti L, Sgaramella V

Abstract

The temperature dependence of the T4 DNA ligase-catalyzed joining of plasmid DNA linearized by the action of HaeIII, EcoRI and PstI restriction endonucleases has been investigated by electron microscopy analysis. The extent of joining is maximal at 4 degrees and decreases with increasing temperatures following sigmoid-like curves. The temperature at which 50% of the maximal reaction is still observable increases going from DNA termini without single-stranded overlaps (produced by HaeIII) to termini with four nucleotides overlap, composed only by two A and two T (produced by EcoRI) to termini with four nucleotide overlap, composed by A, T, G and C (produced by PstI).

MeSH Terms

DNA Ligases/metabolism DNA Restriction Enzymes/metabolism Genes, Viral Kinetics Microscopy, Electron Plasmids Polynucleotide Ligases/metabolism T-Phages/enzymology Temperature

Chemicals

DNA Restriction Enzymes DNA Ligases Polynucleotide Ligases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ferretti L

Sgaramella V

References (15)

15 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/9.1.85

Published

1981-01-10

Pages

85-93

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326670

Subset

External Links

PubMed source DOI: 10.1093/nar/9.1.85 PMC Full Text View journal details

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Temperature dependence of the joining by T4 DNA ligase of termini produced by type II restriction endonucleases.

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