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PMID: 6258165 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A sequence-specific endonuclease (Xmn I) from Xanthomonas manihotis.

Nucleic acids research ·Vol. 8 ·No. 24 ·1980-12-20 ·Pages 6189-98

Lin BC, Chien MC, Lou SY

Abstract

A type II restriction endonuclease Xmn I with a novel site specificity has been isolated from Xanthomonas manihotis. Xmn I does not cleave SV40 DNA, but cleaves phi X174 DNA into three fragments, which constitute 76.61%, 18.08% and 5.31% of the total length of 5386 base pairs, and cleaves pBR322 DNA into two fragments of 55.71% and 44.29% of the entire 4362 base pairs. The nucleotide sequences around the cleavage sites made by Xmn I are not exactly homologous, but they have a common sequence of 5' GAANNNNTTC 3' according to a simple computer program analysis on nucleotide sequences of phi X174 DNA, pBR322 DNA and SV40 DNA. The results suggest that the cleavage site of Xmn I is located within its recognition sequence of 5' GAANNNNTTC 3'.

MeSH Terms
Bacteriophage phi X 174 Base Sequence DNA Restriction Enzymes/metabolism DNA, Viral/metabolism Deoxyribonucleases, Type II Site-Specific Escherichia coli/genetics Plasmids Simian virus 40 Substrate Specificity Xanthomonas/enzymology
Chemicals
DNA, Viral DNA Restriction Enzymes endodeoxyribonuclease XmnI Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lin B C
Chien M C
Lou S Y
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.24.6189
Published
1980-12-20
Pages
6189-98
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328081
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/8.24.6189 PMC Full Text View journal details
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