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PMID: 6330698 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals.

Nucleic acids research ·Vol. 12 ·No. 12 ·1984-06-25 ·Pages 5123-41

Semler BL, Dorner AJ, Wimmer E

Abstract

Sub-genomic cDNA clones representing the entire genomic RNA of poliovirus Type 1 (Mahoney) have been isolated in E. coli. Construction of a complete cDNA copy of the poliovirus genome in the EcoRI site of plasmid vector pBR325 from these clones is described. Introduction of plasmid DNA containing the complete cDNA copy of polio RNA into cultured primate cells by transfection produces infectious poliovirus. The virus produced by such a transfection appears to be identical to wild type poliovirus. Isolation of a polio recombinant plasmid containing SV40 transcription and replication signals is also described. Transfection of COS-1 cells with this plasmid yields greater than 1,600 plaque-forming units (PFU) per microgram of input DNA.

MeSH Terms
Base Sequence Cell Line Cloning, Molecular DNA/metabolism DNA Replication DNA Restriction Enzymes DNA, Viral/genetics Escherichia coli/genetics Genes, Viral Humans Nucleic Acid Conformation Plasmids Poliovirus/genetics Simian virus 40/genetics Transcription, Genetic Transfection
Chemicals
DNA, Viral DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Semler B L
Dorner A J
Wimmer E
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46 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.12.5123
Published
1984-06-25
Pages
5123-41
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318905
Subset
IM
Grants
NIAID NIH HHS · AI 05935 · United States
NIAID NIH HHS · AI 15122 · United States
NCI NIH HHS · CA 28146 · United States
External Links
PubMed source DOI: 10.1093/nar/12.12.5123 PMC Full Text View journal details
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