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PMID: 59925 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 7 ·1976-07-00 ·Pages 2191-5

Kacian DL, Myers JC

Abstract

The synthesis of large, possibly complete, complementary DNA (cDNA) copies of poliovirus RNA by avian myeloblastosis virus DNA polymerase is described. The cDNA consists of two size classes, the larger of which is approximately 7500 nucleotides. In the presence of excess deoxynucleoside triphosphates, ribonucleoside triphosphates, or sodium pyrophosphate, only the larger material is obtained. Yields of the large cDNA are 50-75% of the input RNA.

MeSH Terms
Avian Myeloblastosis Virus/enzymology Cell-Free System DNA, Viral/biosynthesis Deoxyribonucleotides Diphosphates Kinetics Molecular Weight Poliovirus RNA, Viral/metabolism RNA-Directed DNA Polymerase/metabolism Ribonucleotides
Chemicals
DNA, Viral Deoxyribonucleotides Diphosphates RNA, Viral Ribonucleotides RNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kacian D L
Myers J C
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.7.2191
Published
1976-07-00
Pages
2191-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430492
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.7.2191 PMC Full Text View journal details
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