Home LiteratureArticle Details
PMID: 4373729 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Isolation of a viral polypeptide associated with poliovirus RNA polymerase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 12 ·1974-12-00 ·Pages 4773-7

Lundquist RE, Ehrenfeld E, Maizel JV

Abstract

Poliovirus-infected HeLa cells were labeled with radioactive methionine or phenylalanine and subjected to a new purification procedure for the viral induced RNA polymerase activity. Detergent-solubilized polymerase activity was purified by precipitation with 2 M LiCl and sedimentation through sucrose gradients. Approximately 0.001% of the incorporated amino acid radio-activity sediments with the peak of polymerase activity. Gradient fractions comprising the polymerase activity peak were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found to contain predominantly one virus-specific polypeptide. Polyacrylamide gel electrophoresis also reveals that this purified polypeptide migrates with a 58,000 molecular weight noncapsid polio-virus polypeptide.

MeSH Terms
Centrifugation, Density Gradient DNA-Directed RNA Polymerases/isolation & purification Electrophoresis, Polyacrylamide Gel Female HeLa Cells Humans Methionine/metabolism Molecular Weight Peptides/isolation & purification Phenylalanine/metabolism Poliovirus/enzymology Sulfur Radioisotopes Tritium
Chemicals
Peptides Sulfur Radioisotopes Tritium Phenylalanine Methionine DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lundquist R E
Ehrenfeld E
Maizel J V
References (23)
23 references, click to expand
  1. Effects on host cell metabolism following synchronous infection with poliovirus.
    Virology. 1965 Dec;27(4):614-20 PMID: 4159191
  2. STUDIES ON EMC VIRAL RNA SYNTHESIS AND ITS LOCALIZATION IN INFECTED KREBS ASCITES CELLS.
    Virology. 1965 Jul;26:450-65 PMID: 14319717
  3. Aspects of the synthesis of poliovirus RNA and the formation of virus particles.
    Virology. 1966 Jun;29(2):179-89 PMID: 4287327
  4. Evidence for large precursor proteins in poliovirus synthesis.
    Proc Natl Acad Sci U S A. 1968 Mar;59(3):966-71 PMID: 4296044
  5. Action of guanidine on the replication of poliovirus RNA.
    Virology. 1968 Jul;35(3):408-17 PMID: 4298649
  6. Specific cleavage of viral proteins as steps in the synthesis and maturation of enteroviruses.
    Proc Natl Acad Sci U S A. 1968 Jul;60(3):1015-22 PMID: 4299264
  7. Evidence for differences in size and composition of the poliovirus-specific polypeptides in infected HeLa cells.
    Virology. 1968 Sep;36(1):48-54 PMID: 4299390
  8. Polypeptide cleavages in the formation of poliovirus proteins.
    Proc Natl Acad Sci U S A. 1968 Sep;61(1):77-84 PMID: 4301595
  9. The isolation of two enzyme-ribonucleic acid complexes involved in the synthesis of foot-and-mouth disease virus ribonucleic acid.
    Proc Natl Acad Sci U S A. 1969 Mar;62(3):821-8 PMID: 4308096
  10. Membranous structures associated with translation and transcription of poliovirus RNA.
    Science. 1969 Nov 14;166(3907):885-6 PMID: 4310189
  11. Soluble RNA polymerase complex from poliovirus-infected HeLa cells.
    Virology. 1970 Apr;40(4):840-6 PMID: 4317360
  12. Subunit structure of Q-beta replicase.
    Nature. 1970 Nov 7;228(5271):525-7 PMID: 5472467
  13. Characterization of the subunits of Q-beta replicase.
    Nature. 1970 Nov 7;228(5271):527-33 PMID: 5472468
  14. Comparative aspects of Poliovirus replication.
    J Cell Physiol. 1970 Dec;76(3):265-71 PMID: 4322979
  15. Determination of the gene sequence of poliovirus with pactamycin.
    Proc Natl Acad Sci U S A. 1971 Nov;68(11):2852-6 PMID: 4330946
  16. Localization of Q-beta maturation cistron ribosome binding site.
    Nat New Biol. 1971 Sep 15;234(50):202-4 PMID: 5288805
  17. Proteins associated with the poliovirus RNA replication complex.
    Virology. 1971 Nov;46(2):375-86 PMID: 4331730
  18. RNA polymerase complexes from mengovirus infected cells.
    Arch Gesamte Virusforsch. 1972;38(1):17-28 PMID: 4341300
  19. A comparison of the virus-specific polypeptides of encephalomyocarditis virus, human rhinovirus-1A, and poliovirus.
    Virology. 1973 Dec;56(2):439-53 PMID: 4357051
  20. Poliovirus-induced RNA polymerase and the effects of virus-specific inhibitors on its production.
    Proc Natl Acad Sci U S A. 1963 Jun;49:843-9 PMID: 13966259
  21. A SIMPLE METHOD FOR THE QUANTITATIVE ISOLATION OF UNDEGRADED HIGH MOLECULAR WEIGHT RIBONUCLEIC ACID.
    Biochem Biophys Res Commun. 1963 Sep 10;13:61-6 PMID: 14069514
  22. IN VITRO SYNTHESIS OF VIRAL RNA BY THE POLIOVIRUS RNA POLYMERASE.
    Proc Natl Acad Sci U S A. 1964 Mar;51:450-6 PMID: 14171458
  23. Symposium on replication of viral nucleic acids. 3. Replication of mengovirus ribonucleic acid.
    Bacteriol Rev. 1966 Jun;30(2):288-308 PMID: 4287031
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.12.4773
Published
1974-12-00
Pages
4773-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433979
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.12.4773 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2