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PMID: 6310508 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence of poliovirus type 3 leon 12 a1b: comparison with poliovirus type 1.

Nucleic acids research ·Vol. 11 ·No. 16 ·1983-08-25 ·Pages 5629-43

Stanway G, Cann AJ, Hauptmann R, Hughes P, Clarke LD, Mountford RC, Minor PD, Schild GC, Almond JW

Abstract

The complete nucleotide sequence of the genome of the Sabin vaccine strain of poliovirus type 3 (P3/Leon 12 a1 b) has been determined from cDNA cloned in E. coli. The genome comprises a 5' non-coding region of 742 nucleotides, a large open reading frame of 6618 nucleotides (89% of the sequence) and a 3' non-coding region of 72 nucleotides. There is 77.4% base-sequence homology and 89.6% predicted amino-acid homology between types 1 and 3. Conservation of all glutamine-glycine and tyrosine-glycine cleavage sites suggests a mechanism of polyprotein processing similar to that established for poliovirus type 1.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/analysis Genes Genes, Viral Poliovirus/genetics Poliovirus Vaccine, Oral Species Specificity
Chemicals
Poliovirus Vaccine, Oral DNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Stanway G
Cann A J
Hauptmann R
Hughes P
Clarke L D
Mountford R C
Minor P D
Schild G C
Almond J W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.16.5629
Published
1983-08-25
Pages
5629-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326302
Subset
IM
Databases
GENBANK
K00043, X00925, X01076
External Links
PubMed source DOI: 10.1093/nar/11.16.5629 PMC Full Text View journal details
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