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PMID: 6260985 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Stable hairpin structure within the 5'-terminal 85 nucleotides of poliovirus RNA.

Journal of virology ·Vol. 37 ·No. 1 ·1981-01-00 ·Pages 328-35

Larsen GR, Semler BL, Wimmer E

Abstract

The primary sequence of a 5'-terminal fragment of poliovirus type 1 RNA, generated by digestion with RNase III, has been determined. This sequence reveals the presence of a stable hairpin structure beginning nine nucleotides from the terminally linked protein VPg. The sequence does not contain (i) the initiation codons AUG or GUG or (ii) the putative ribosome-binding sequence complementary to the 3' end of eucaryotic ribosomal 18S RNA. The stem-and-loop structure identified can be drawn in either plus or minus RNA strands. It is unclear to which strand functional significance (if any) can be assigned. It is possible that the hairpin structure is involved in ribosomal recognition and translation or in RNA synthesis by interacting with replicase molecules.

MeSH Terms
Base Sequence Nucleic Acid Conformation Poliovirus/genetics RNA, Messenger RNA, Viral Ribonuclease T1 Ribonucleases
Chemicals
RNA, Messenger RNA, Viral Ribonucleases Ribonuclease T1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Larsen G R
Semler B L
Wimmer E
References (47)
47 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.37.1.328-335.1981
Published
1981-01-00
Pages
328-35
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171010
Subset
IM
Grants
NIAID NIH HHS · AI 15122 · United States
Databases
GENBANK
J02281
External Links
PubMed source DOI: 10.1128/JVI.37.1.328-335.1981 PMC Full Text View journal details
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