Home LiteratureArticle Details
PMID: 6253931 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The structure of poliovirus replicative form.

Nucleic acids research ·Vol. 8 ·No. 6 ·1980-03-25 ·Pages 1217-29

Larsen GR, Dorner AJ, Harris TJ, Wimmer E

Abstract

The structure of polio replicative form (RF) has been investigated by 3' end labeling and the use of polynucleotide phosphorylase to now allow a complete composite of the RF structure. The evidence presented indicates that the 3' terminal sequence of the minus strand is an exact complement to the 5' end of polio RNA. This suggests that the 5' terminal U of polio RNA is genetically coded. Other data is presented to show that in addition to the genetically coded poly(A) tract of the plus strand in RF, a single-stranded poly(A) tail protrudes beyond the double-stranded RNA.

MeSH Terms
Base Sequence Genes, Viral Nucleic Acid Conformation Poliovirus/genetics Poly A/genetics Polyribonucleotide Nucleotidyltransferase RNA, Double-Stranded/genetics RNA, Viral/genetics Virus Replication
Chemicals
RNA, Double-Stranded RNA, Viral Poly A Polyribonucleotide Nucleotidyltransferase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Larsen G R
Dorner A J
Harris T J
Wimmer E
References (28)
28 references, click to expand
  1. Purification and properties of poliovirus double-stranded ribonucleic acid.
    J Mol Biol. 1966 Jul;18(3):421-8 PMID: 4290740
  2. Double-stranded poliovirus RNA inhibits initiation of protein synthesis by reticulocyte lysates.
    Proc Natl Acad Sci U S A. 1971 May;68(5):1075-8 PMID: 4325000
  3. Polyadenylic acid at the 3'-terminus of poliovirus RNA.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1877-82 PMID: 4340162
  4. Poly (A) and poly (U) in poliovirus double stranded RNA.
    Nat New Biol. 1973 Apr 11;242(119):171-4 PMID: 4349601
  5. Requirement of 3'-terminal poly(adenylic acid) for the infectivity of poliovirus RNA.
    Proc Natl Acad Sci U S A. 1974 Aug;71(8):2983-7 PMID: 4370340
  6. Poly(U) in poliovirus minus RNA is 5'-terminal.
    Biochem Biophys Res Commun. 1974 Dec 23;61(4):1101-9 PMID: 4376003
  7. Polyadenylic acid on poliovirus RNA. II. poly(A) on intracellular RNAs.
    J Virol. 1975 Jun;15(6):1418-31 PMID: 167193
  8. Polyadenylic acid on poliovirus RNA IV. Poly(U) in replicative intermediate and double-stranded RNA.
    Virology. 1975 Oct;67(2):498-505 PMID: 171838
  9. Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded.
    J Virol. 1975 Dec;16(6):1512-7 PMID: 172656
  10. A protein covalently linked to poliovirus genome RNA.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):59-63 PMID: 189316
  11. Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.
    Proc Natl Acad Sci U S A. 1977 Mar;74(3):961-5 PMID: 191841
  12. The location of the polio genome protein in viral RNAs and its implication for RNA synthesis.
    Nature. 1977 Jul 21;268(5617):208-13 PMID: 196204
  13. Mapping adenines, guanines, and pyrimidines in RNA.
    Nucleic Acids Res. 1977 Aug;4(8):2527-38 PMID: 409999
  14. The use of nuclease P1 in sequence analysis of end group labeled RNA.
    Nucleic Acids Res. 1977 Dec;4(12):4091-108 PMID: 202925
  15. The 5'-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5345-9 PMID: 202952
  16. Enzymatic oligoribonucleotide synthesis with T4 RNA ligase.
    Biochemistry. 1978 May 30;17(11):2069-76 PMID: 667012
  17. Homologous nucleotide sequences between prokaryotic and eukaryotic mRNAs: the 5'-end sequence of the mRNA of the lipoprotein of the Escherichia coli outer membrane.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2190-4 PMID: 353808
  18. Identification of a protein linked to nascent poliovirus RNA and to the polyuridylic acid of negative-strand RNA.
    J Virol. 1978 Aug;27(2):357-65 PMID: 211265
  19. 3'-terminal labelling of RNA with T4 RNA ligase.
    Nature. 1978 Oct 12;275(5680):560-1 PMID: 692735
  20. Poliovirus single-stranded RNA and double-stranded RNA: differential infectivity in enucleate cells.
    J Virol. 1978 Sep;27(3):582-6 PMID: 212582
  21. 3'-Terminal nucleotide sequences in the genome RNA of picornaviruses.
    Nature. 1978 Nov 16;276(5685):298-301 PMID: 213732
  22. An electron microscope study of the proteins attached to polio virus RNA and its replicative form (RF).
    Nucleic Acids Res. 1978 Dec;5(12):4711-23 PMID: 218168
  23. Strand-specific attachment of avidin-spheres to double-stranded poliovirus RNA.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):676-80 PMID: 218216
  24. The enzymatic preparation of [alpha-32P]ATP, [alpha-32P]GTP, [32P]cAMP, and [32P]cGMP, and their use in the assay of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterases.
    Adv Cyclic Nucleotide Res. 1979;10:135-67 PMID: 36738
  25. Determination of the length distribution of poly(A) at the 3' terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA.
    Nucleic Acids Res. 1979 Nov 10;7(5):1195-204 PMID: 229465
  26. Sequence studies of poliovirus RNA. IV. Nucleotide sequence complexities of poliovirus type 1, type 2 and two type 1 defective interfering particles RNAs, and fingerprint of the poliovirus type 3 genome.
    J Gen Virol. 1979 Aug;44(2):311-22 PMID: 230285
  27. The nucleotide sequence at the 5' end of foot and mouth disease virus RNA.
    Nucleic Acids Res. 1979 Dec 11;7(7):1765-85 PMID: 231762
  28. INFECTIOUS DOUBLE-STRANDED POLIOVIRUS RNA.
    Virology. 1964 Nov;24:467-73 PMID: 14231594
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.6.1217
Published
1980-03-25
Pages
1217-29
Language
English
Region
England
NLM ID
0411011
PMCID
PMC323987
Subset
IM
Grants
NIAID NIH HHS · AI-15122 · United States
NCI NIH HHS · CM-07212 · United States
External Links
PubMed source DOI: 10.1093/nar/8.6.1217 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2