A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.

The EMBO journal ·Vol. 3 ·No. 4 ·1984-04-00 ·Pages 761-6

Hirochika H, Nakamura K, Sakaguchi K

Abstract

The terminal structure of a linear plasmid pSLA2 , which was isolated from Streptomyces rochei , was analysed. The 5' ends of pSLA2 DNA were blocked by the association of a protein probably covalently bonded with the DNA. This block is removed by alkali treatment and blunt ends with 5'-phosphate and 3'-hydroxy termini were released. The two terminal fragments of pSLA2 were cloned and the nucleotide sequence was determined. An inverted terminal repetition of 614 bp was found along with the presence of further interrupted homologous sequences beyond this area up to 800 bp. These are the first inverted terminal repeat sequences found in microbial linear plasmids.

MeSH Terms

Base Composition Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial Plasmids Streptomyces/genetics

Chemicals

DNA, Bacterial DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hirochika H

Nakamura K

Sakaguchi K

References (31)

31 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1984-04-00

Pages

761-6

Language

English

Region

England

NLM ID

8208664

PMCID

PMC557423

Subset

Databases

GENBANK

X00500, X00511

External Links

PubMed source PMC Full Text View journal details

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A linear DNA plasmid from Streptomyces rochei with an inverted terminal repetition of 614 base pairs.

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