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PMID: 6262789 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequences at the termini of phi 29 DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1336-40

Yoshikawa H, Friedmann T, Ito J

Abstract

The nucleotide sequences of the first 422 base pairs from the left-hand end and the first 274 base pairs from the right-hand end of phi 29 DNA were determined by using the chemical degradation method of Maxam and Gilbert. The data indicate that phi 29 DNA has inverted terminal repetitions that are six base pairs long 5' (-A-A-A-G-T-A-). No perfectly self-complementary sequence exists within the terminal regions of phi 29 DNA, suggesting that DNA replication via a self-priming mechanism is improbable. The putative early promoter sequences were found in both ends of the phi 29 DNA. The results of the sequence determination are discussed in relation ship to models proposed for the mechanism of replication of linear DNA molecules.

MeSH Terms
Base Composition Base Sequence Coliphages/analysis DNA Restriction Enzymes DNA, Viral
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yoshikawa H
Friedmann T
Ito J
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42 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1336
Published
1981-03-00
Pages
1336-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319125
Subset
IM
Grants
NIGMS NIH HHS · GM28013 · United States
NIGMS NIH HHS · GM28362 · United States
Databases
GENBANK
J02477, J02481
External Links
PubMed source DOI: 10.1073/pnas.78.3.1336 PMC Full Text View journal details
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