Home LiteratureArticle Details
PMID: 6262800 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage phi 29.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1446-50

Escarmís C, Salas M

Abstract

Phage phi 29 DNA cannot be phosphorylated with polynucleotide kinase and [gamma-32P]ATP because of the presence of a viral protein covalently linked to the 5' termini. The 5' ends can, however, be made susceptible to phosphorylation by treatment with alkali and alkaline phosphatase. Restriction fragments Hpa II C and Hpa II F, corresponding to the right and left ends of phi 29 DNA, respectively, were labeled at the 5' ends with polynucleotide kinase and [gamma-32P]ATP or at the 3' ends with terminal transferase and [alpha-32P]ATP or [alpha-32P]cordycepin 5'-triphosphate. After a secondary cleavage of the labeled fragments, the sequence of the first 150-180 nucleotides at the termini of phi 29 DNA was determined by the method of Maxam and Gilbert. The ends of phi 29 DNA are flush, and a six-nucleotides-long inverted terminal repetition was found. The functional implications of the sequences determined are discussed.

MeSH Terms
Alkaline Phosphatase Bacillus subtilis/analysis Bacteriophages/analysis Base Sequence DNA Restriction Enzymes DNA, Viral Oligodeoxyribonucleotides/analysis
Chemicals
DNA, Viral Oligodeoxyribonucleotides DNA Restriction Enzymes Alkaline Phosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Escarmís C
Salas M
References (50)
50 references, click to expand
  1. The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.
    Biochem J. 1969 Jun;113(1):131-8 PMID: 4896715
  2. Nucleotide sequences at the termini of phi 29 DNA.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1336-40 PMID: 6262789
  3. Proteins induced in Bacillus subtilis infected with bacteriophage phi 29.
    Virology. 1973 Nov;56(1):291-9 PMID: 4200881
  4. The synthesis and purification of (gamma-32P)-adenosine triphosphate with high specific activity.
    J Biol Chem. 1973 Dec 10;248(23):8319-21 PMID: 4752959
  5. Viral protein synthesis in bacteriophage phi 29-infected Bacillus subtilis.
    J Virol. 1973 Nov;12(5):1149-59 PMID: 4203085
  6. Reversal of bacteriophage T4 induced polynucleotide kinase action.
    Biochemistry. 1973 Dec 4;12(25):5050-5 PMID: 4366306
  7. Determinant of cistron specificity in bacterial ribosomes.
    Nature. 1975 Mar 6;254(5495):34-8 PMID: 803646
  8. Palindromic base sequences and replication of eukaryote chromosome ends.
    Nature. 1974 Aug 9;250(5466):467-70 PMID: 4469597
  9. Genetic analysis of bacteriophage phi 29 of Bacillus subtilis: integration and mapping of reference mutants of two collections.
    J Virol. 1976 Aug;19(2):495-500 PMID: 822174
  10. Physical map of bacteriophage phi29 DNA.
    Virology. 1976 Oct 15;74(2):314-23 PMID: 824814
  11. Evidence for blocked 5'-termini in human adenovirus DNA.
    Virology. 1977 Jan;76(1):380-94 PMID: 835234
  12. Studies on the mechanism of replication of adenovirus DNA. V. The location of termini of replication.
    Virology. 1977 Mar;77(1):149-57 PMID: 851468
  13. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  14. Initiation and termination sites of adenovirus 12 DNA replication.
    Virology. 1977 May 15;78(2):415-24 PMID: 867811
  15. Identification of a protein linked to the ends of adenovirus DNA.
    Cell. 1977 Jun;11(2):283-95 PMID: 196758
  16. The structure of replicating adenovirus 2 DNA molecules.
    Cell. 1977 Dec;12(4):1007-20 PMID: 597856
  17. The nucleotide sequence at the termini of adenovirus type 5 DNA.
    Nucleic Acids Res. 1977 Dec;4(12):4371-89 PMID: 600799
  18. Identification of the protein firmly bound to the ends of bacteriophage phi 29 DNA.
    Virology. 1978 Feb;84(2):279-92 PMID: 203093
  19. The mechanism of replication of adenovirus DNA. VI. Localization of the origins of the displacement synthesis.
    Virology. 1978 Feb;84(2):509-17 PMID: 622809
  20. Characterization of a protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi29.
    J Mol Biol. 1978 Feb 25;119(2):269-91 PMID: 416224
  21. Genome-linked protein associated with the 5' termini of bacteriophage phi29 DNA.
    J Virol. 1978 Sep;27(3):776-83 PMID: 100608
  22. Bacteriophage phi29 terminal protein: its association with the 5' termini of the phi29 genome.
    J Virol. 1978 Dec;28(3):895-904 PMID: 731797
  23. The DNA sequence at the T7 C promoter.
    Nucleic Acids Res. 1979 Feb;6(2):525-44 PMID: 370790
  24. RNA polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage phi29.
    J Mol Biol. 1979 Feb 5;127(4):411-36 PMID: 107317
  25. An adenovirus protein associated with the ends of replicating DNA molecules.
    Virology. 1979 Feb;93(1):69-79 PMID: 433158
  26. The nucleotide sequences at the termini of adenovirus-2 DNA.
    J Mol Biol. 1979 Mar 15;128(4):577-94 PMID: 318164
  27. Nucleotide sequence at the inverted terminal repetition of adenovirus type 2 DNA.
    Biochem Biophys Res Commun. 1979 Apr 13;87(3):671-8 PMID: 454416
  28. Replicating adenovirus 2 DNA molecules contain terminal protein.
    Virology. 1979 Jul 15;96(1):143-58 PMID: 462806
  29. Sequence of inverted terminal repetitions from different adenoviruses: demonstration of conserved sequences and homology between SA7 termini and SV40 DNA.
    Cell. 1979 Jul;17(3):705-13 PMID: 225041
  30. Origin of replication, oriC, of the Escherichia coli K12 chromosome: nucleotide sequence.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:139-45 PMID: 157832
  31. Dissection and comparative anatomy of the origins of replication of lambdoid phages.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:155-63 PMID: 157834
  32. Functional analysis of the replicator structure of lambdoid bacteriophage DNAs.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 1:165-78 PMID: 157835
  33. Nucleotide sequence of the mitochondrial structural genes for cysteine-tRNA and histidine-tRNA of yeast.
    Nucleic Acids Res. 1979 Jul 25;6(10):3255-66 PMID: 384365
  34. The structure of replicating adenovirus DNA molecules: characterization of DNA-protein complexes from infected cells.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:721-8 PMID: 226316
  35. Comparison of the A-T rich regions and the Bacillus subtilis RNA polymerase binding sites in phage phi 29 DNA.
    Nucleic Acids Res. 1979 Sep 11;7(1):107-20 PMID: 114982
  36. The nucleotide sequence of the right-hand terminus of adenovirus type 5 DNA: implications for the mechanism of DNA replication.
    Gene. 1979 Aug;6(4):307-18 PMID: 264242
  37. Specific cleavage of the terminal protein from the adenovirus 5 DNA under the condition of single-strand scission by nuclease S1.
    FEBS Lett. 1979 Nov 15;107(2):355-8 PMID: 510545
  38. Distinctive nucleotide sequences of promoters recognized by RNA polymerase containing a phage-coded "sigma-like" protein.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5465-9 PMID: 118447
  39. The inverted terminal repetition of the DNA of weakly oncogenic adenovirus type 7.
    Gene. 1979 Dec;8(1):7-15 PMID: 231543
  40. Structure of replicating DNA molecules of Bacillus subtilis bacteriophage phi 29.
    J Virol. 1980 Apr;34(1):187-99 PMID: 6768899
  41. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  42. 3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate.
    Gene. 1980 Jul;10(2):177-83 PMID: 6248422
  43. The protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi 29 is involved in the initiation of DNA replication.
    Virology. 1980 Jul 15;104(1):84-96 PMID: 6771916
  44. DNA replication of bacteriophage phi 29: characterization of the intermediates and location of the termini of replication.
    Virology. 1980 Jul 30;104(2):323-38 PMID: 7395108
  45. The nucleotide sequence of a cloned human leukocyte interferon cDNA.
    Gene. 1980 Jun;10(1):1-10 PMID: 6157600
  46. Initiation of adenovirus DNA replication.
    J Virol. 1980 Sep;35(3):662-71 PMID: 7420538
  47. Assignment of reovirus mRNA ribosome binding sites to virion genome segments by nucleotide sequence analyses.
    Nucleic Acids Res. 1980 Jan 25;8(2):337-50 PMID: 7422544
  48. Comparative sequence analysis of the inverted terminal repetitions from different adenoviruses.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3831-5 PMID: 6253991
  49. Protein p3 is linked to the DNA of phage phi 29 through a phosphoester bond between serine and 5'-dAMP.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6425-8 PMID: 6779279
  50. Nucleotide sequence analysis of deoxyribonucleic acid. VI. Determination of 3'-terminal dnucleotide sequences of several species of duplex deoxyribonucleic acid using Escherichia coli deoxyribonucleic acid polymerase I.
    J Biol Chem. 1972 Jul 25;247(14):4654-60 PMID: 4557849
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1446
Published
1981-03-00
Pages
1446-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319147
Subset
IM
Grants
NIGMS NIH HHS · 1 R01 GM27242-01 · United States
Databases
GENBANK
J02477, J02481
External Links
PubMed source DOI: 10.1073/pnas.78.3.1446 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2