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PMID: 6402761 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Factors involved in the initiation of phage phi 29 DNA replication in vitro: requirement of the gene 2 product for the formation of the protein p3-dAMP complex.

Nucleic acids research ·Vol. 11 ·No. 5 ·1983-03-11 ·Pages 1309-23

Blanco L, Garcìa JA, Peñalva MA, Salas M

Abstract

To study the requirements for the in vitro formation of the protein p3-dAMP complex, the first step in phi29 DNA replication, extracts from B. subtilis infected with phi29 mutants in genes 2, 3, 5, 6 and 17, involved in DNA synthesis, have been used. The formation of the initiation complex is completely dependent on the presence of a functional gene 2 product, in addition to protein p3 and phi29 DNA-protein p3 as template. ATP is also required, although it can be replaced by other nucleotides. The products of genes 5, 6 and 17 do not seem to be needed in the formation of the initiation complex. Inhibitors of the host DNA polymerase III, DNA gyrase or RNA polymerase had no effect on the formation of the protein p3-dAMP complex, suggesting that these proteins are not involved in the initiation of phi29 DNA replication. ddATP or aphidicolin, inhibitors of DNA chain elongation, had also no effect on the formation of the initiation complex.

MeSH Terms
Adenosine Diphosphate/pharmacology Bacillus subtilis/genetics Bacteriophages/genetics DNA Replication Deoxyribonucleotides/pharmacology Genes Genes, Viral Mutation Peptide Chain Initiation, Translational Protein Biosynthesis/drug effects Ribonucleotides/pharmacology Transcription, Genetic/drug effects Viral Proteins/genetics Virus Replication
Chemicals
Deoxyribonucleotides Ribonucleotides Viral Proteins Adenosine Diphosphate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Blanco L
Garcìa J A
Peñalva M A
Salas M
References (41)
41 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.5.1309
Published
1983-03-11
Pages
1309-23
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325798
Subset
IM
Grants
NIGMS NIH HHS · 1 R01 GM27242-03 · United States
External Links
PubMed source DOI: 10.1093/nar/11.5.1309 PMC Full Text View journal details
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