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PMID: 6957861 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Separation of the adenovirus terminal protein precursor from its associated DNA polymerase: role of both proteins in the initiation of adenovirus DNA replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 17 ·1982-09-00 ·Pages 5225-9

Lichy JH, Field J, Horwitz MS, Hurwitz J

Abstract

A complex containing the 80,000-dalton precursor to the adenovirus (Ad)-encoded terminal protein (pTP) and a 140,000-dalton protein is required for Ad DNA replication in vitro. This complex has been separated into subunits by glycerol gradient centrifugation in the presence of urea. The isolated 140,000-dalton subunit contains a DNA polymerase activity which can be differentiated from all host DNA polymerases. No enzyme activity was detected with the isolated pTP. The requirements for reactions involved in the initiation of Ad DNA replication were determined by using the isolated subunits. The covalent addition of dCMP, the first nucleotide in the DNA chain, to the pTP, which serves as the primer for replication, required the DNA polymerase subunit as well as the pTP. Synthesis of viral DNA in vitro also required both subunits. The properties of the DNA polymerase suggest that it may be a viral gene product.

MeSH Terms

Adenoviruses, Human/metabolism DNA Replication DNA, Viral/genetics DNA-Directed DNA Polymerase/metabolism HeLa Cells/metabolism Kinetics Macromolecular Substances Protein Precursors/isolation & purification,metabolism Viral Proteins/isolation & purification,metabolism Virus Replication

Chemicals

DNA, Viral Macromolecular Substances Protein Precursors Viral Proteins DNA-Directed DNA Polymerase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lichy J H

Field J

Horwitz M S

Hurwitz J

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22 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.17.5225

Published

1982-09-00

Pages

5225-9

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC346868

Subset

External Links

PubMed source DOI: 10.1073/pnas.79.17.5225 PMC Full Text View journal details

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Separation of the adenovirus terminal protein precursor from its associated DNA polymerase: role of both proteins in the initiation of adenovirus DNA replication.

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