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PMID: 482131 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The effect of aphidicolin on adenovirus DNA synthesis.

Nucleic acids research ·Vol. 6 ·No. 10 ·1979-07-25 ·Pages 3369-86

Longiaru M, Ikeda JE, Jarkovsky Z, Horwitz SB, Horwitz MS

Abstract

Aphidicolin inhibits adenovirus DNA replication in HeLa cells and in a cell-free, infected, nuclear extract in which viral DNA is elongated. The compound inhibits alpha DNA polymerase, extensively purified from HeLa cells, but has little or no effect on the beta or gamma DNA polymerases similarly purified. Aphidicolin does not affect thymidine uptake by cells nor does synthesis as it also inhibits DNA replication in uninfected cells. The inhibition by aphidicolin is reversible if the drug is removed within 18 hrs after addition to HeLa or Chinese Hamster Ovary cells but the cells are irreversibly affected if the drug remains for 48 hours.

MeSH Terms

Adenoviruses, Human/metabolism Animals Anti-Bacterial Agents/pharmacology Cell Line Cell Nucleus/metabolism Cricetinae DNA Replication/drug effects DNA, Neoplasm/metabolism DNA, Viral/biosynthesis DNA-Directed DNA Polymerase/metabolism Diterpenes/pharmacology Female HeLa Cells/drug effects,enzymology Humans Ovary Thymidine/metabolism

Chemicals

Anti-Bacterial Agents DNA, Neoplasm DNA, Viral Diterpenes DNA-Directed DNA Polymerase Thymidine

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Longiaru M

Ikeda J E

Jarkovsky Z

Horwitz S B

Horwitz M S

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26 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/6.10.3369

Published

1979-07-25

Pages

3369-86

Language

English

Region

England

NLM ID

0411011

PMCID

PMC327939

Subset

External Links

PubMed source DOI: 10.1093/nar/6.10.3369 PMC Full Text View journal details

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