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PMID: 6269087 Published · ppublish English Journal Article

The integrated forms of the S1 and S2 DNA elements of maize male sterile mitochondrial DNA are flanked by a large repeated sequence.

Nucleic acids research ·Vol. 9 ·No. 15 ·1981-08-11 ·Pages 3657-69

Lonsdale DM, Thompson RD, Hodge TP

Abstract

The mitochondrial DNA of maize was cloned using the cosmid, Homer I. Recombinants carrying sequences homologous to the S1 and S2 DNA elements of male sterile maize have been analysed. Restriction endonuclease maps for Sac II, Sma I and Bam HI have been constructed. The S1 and S2 sequences are single copy sequences occurring at unique sites; each is flanked by a 26 kb repeated sequence. The repeated sequence has been shown not to contain the mitochondrial ribosomal RNA genes.

MeSH Terms
Base Sequence Cloning, Molecular DNA Replication DNA Restriction Enzymes DNA, Mitochondrial/genetics DNA, Recombinant/metabolism Genetic Vectors Plants/genetics Plasmids Zea mays/genetics
Chemicals
DNA, Mitochondrial DNA, Recombinant DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lonsdale D M
Thompson R D
Hodge T P
References (19)
19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1981-08-11
Pages
3657-69
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327382
Subset
IM
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