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PMID: 6324180 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Resistance of human cells to tumorigenesis induced by cloned transforming genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 24 ·1983-12-00 ·Pages 7601-5

Sager R, Tanaka K, Lau CC, Ebina Y, Anisowicz A

Abstract

The transformation of human cells was examined by transfection of cloned oncogenic DNAs derived from the tumor virus simian virus 40 and from the human bladder carcinoma cell line EJ into diploid fibroblasts derived from foreskin (FS-2 cells). The simian virus 40 DNA was found to induce a morphologically transformed phenotype, leading to easily detectable focus formation. Tumor antigen was produced, but the transformed cells were not tumorigenic in the nude mouse. The EJ gene, a mutant form of the cellular c-Ha-ras gene, actively transforms NIH/3T3 mouse cells and CHEF/18 hamster cells but is inactive in FS-2 cells. Morphological transformation, focus formation, and tumorigenicity in nude mice were not induced when EJ DNA was transfected into FS-2 cells by using the selectable vector pSVgptEJ. The intactness of the transfected EJ DNA was established by restriction fragment analysis. This result raises the question of what role, if any, the mutated gene derived from the EJ cells played in the origin of the EJ bladder carcinoma.

MeSH Terms
Animals Base Sequence Cell Line Cell Transformation, Neoplastic Cloning, Molecular Cricetinae Cricetulus DNA Restriction Enzymes DNA, Neoplasm/genetics Drug Resistance Humans Mice Mice, Nude Neoplasm Transplantation Oncogenes Simian virus 40/genetics Transfection Transplantation, Heterologous
Chemicals
DNA, Neoplasm DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sager R
Tanaka K
Lau C C
Ebina Y
Anisowicz A
References (31)
31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.24.7601
Published
1983-12-00
Pages
7601-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC534388
Subset
IM
Grants
NCI NIH HHS · CA22427 · United States
NCI NIH HHS · CA24828 · United States
External Links
PubMed source DOI: 10.1073/pnas.80.24.7601 PMC Full Text View journal details
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