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PMID: 6322125 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein.

Nucleic acids research ·Vol. 12 ·No. 4 ·1984-02-24 ·Pages 1875-87

Estes MK, Mason BB, Crawford S, Cohen J

Abstract

The nucleotide sequence of the gene that codes for the major inner capsid protein of the simian rotavirus SA11 has been determined. A DNA copy of mRNA from gene 6 was cloned in the E. coli plasmid pBR322. The full-length gene is 1357 nucleotides long with a 5'-noncoding region of 23 nucleotides and a 3'-noncoding region of 140 nucleotides. The gene contains a single, long, open reading-frame of 1194 nucleotides capable of coding for a protein of 397 amino acids with a molecular weight of 44,816. The predicted protein product is relatively proline-rich with a net charge at neutral pH of -3.5. One stretch of 53 amino acids (encoded by nucleotides 327-485) is basic.

MeSH Terms
Base Sequence Capsid/genetics,isolation & purification Cloning, Molecular Escherichia coli/genetics Genes Genes, Viral Molecular Weight Plasmids RNA, Messenger/genetics Rotavirus/genetics Viral Proteins/genetics,isolation & purification Viral Structural Proteins
Chemicals
RNA, Messenger Viral Proteins Viral Structural Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Estes M K
Mason B B
Crawford S
Cohen J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.4.1875
Published
1984-02-24
Pages
1875-87
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318627
Subset
IM
Grants
NCI NIH HHS · CA09197 · United States
Databases
GENBANK
X00421
External Links
PubMed source DOI: 10.1093/nar/12.4.1875 PMC Full Text View journal details
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