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PMID: 6293413 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Serotyping and subgrouping of rotavirus strains by the ELISA test.

Archives of virology ·Vol. 73 ·No. 3-4 ·1982-00-00 ·Pages 219-30

Thouless ME, Beards GM, Flewett TH

Abstract

A method is described for the serotyping and subgrouping of rotaviruses by enzyme-linked immuno-sorbent assay (ELISA). For the isolates tested "blind" under code in parallel the serotyping results obtained by ELISA and serum neutralization of fluorescent focus formation were the same. After absorption of the typing antisera with purified heterotypic rotavirus, up to 128-fold differences in titres between isolates were observed in the ELISA test. The results of serotyping and subgrouping, by ELISA, of strains previously described and characterized by the authors' and other laboratories are also given. An attempt is made to correlate the serological findings with variations between virus strains based upon patterns of genome molecular weights as revealed by polyacrylamide gel electrophoresis. The application of this ELISA technique to epidemiological studies and vaccine research is discussed.

MeSH Terms
Animals Cattle Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Humans Molecular Weight Neutralization Tests RNA, Viral/classification Rotavirus/classification Serotyping Sheep Swine
Chemicals
RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Thouless M E
Beards G M
Flewett T H
References (21)
21 references, click to expand
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01318076
Published
1982-00-00
Pages
219-30
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01318076 View journal details
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