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PMID: 6168588 Published · ppublish English Journal Article

Antigenic characterization of human and animal rotaviruses by immune adherence hemagglutination assay (IAHA): evidence for distinctness of IAHA and neutralization antigens.

Infection and immunity ·Vol. 33 ·No. 2 ·1981-08-00 ·Pages 415-25

Kapikian AZ, Cline WL, Greenberg HB, Wyatt RG, Kalica AR, Banks CE, James HD, Flores J, Chanock RM

Abstract

An immune adherence hemagglutination assay (IAHA) and a modified enzyme-linked immunosorbent assay for antigenic characterization of human rotaviruses were developed. The designations of type 1 and type 2 were identical to those established previously by specific complement fixation, enzyme-linked immunosorbent assay, and immune electron microscopy. By IAHA (and modified enzyme-linked immunosorbent assay) certain animal rotaviruses were found to be closely related to human rotavirus type 1. The pattern of IAHA reactivity and the cell culture neutralization serotype were found to be distinct properties. The separation of neutralization and IAHA reactivity was apparent when animal rotaviruses which were distinguishable from each other by neutralization assays were found to share IAHA specificity. Further evidence for the dissociation of the neutralization and IAHA specificities was found in studies of human and bovine rotaviruses which underwent genetic reassortment during coinfection. Thus, it appeared that the IAHA and neutralization antigens were coded for by different genes. In view of these findings, we suggest that the term serotype be reversed to identify the antigen that reacts with neutralizing antibodies as is customary for other viruses and that the term subgroup (instead of serotype) be used for the specificity detected by specific complement fixation, enzyme-linked immunosorbent assay, and now IAHA.

MeSH Terms
Animals Antigens, Viral/analysis Cattle Enzyme-Linked Immunosorbent Assay Epitopes Hemagglutination Tests Humans Immune Adherence Reaction Neutralization Tests Reoviridae/immunology Rotavirus/classification,immunology Serotyping
Chemicals
Antigens, Viral Epitopes
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Kapikian A Z
Cline W L
Greenberg H B
Wyatt R G
Kalica A R
Banks C E
James H D
Flores J
Chanock R M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.33.2.415-425.1981
Published
1981-08-00
Pages
415-25
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC350714
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.33.2.415-425.1981 PMC Full Text View journal details
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