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PMID: 6287021 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Sequence diversity of human rotavirus strains investigated by northern blot hybridization analysis.

Journal of virology ·Vol. 43 ·No. 2 ·1982-08-00 ·Pages 369-78

Street JE, Croxson MC, Chadderton WF, Bellamy AR

Abstract

Rotavirus genomic RNAs, derived from a series of human isolates that exhibit variability in the pattern of migration of the double-stranded RNA on polyacrylamide gels, were transferred to diazobenzyloxymethyl paper, and their sequence diversity was investigated. Hybridization of cDNA probes prepared from the 11 segments of rotavirus RNA indicated that considerable sequence diversity exists among these viruses. Under conditions of both low and high stringency, hybridization analysis of virus collected between 1975 and 1980 suggested that the variation among rotavirus strains may have occurred by a process involving both "drift" and "shift" in the sequence of the rotavirus genomic segments.

MeSH Terms
Base Sequence DNA Genes, Viral Humans Nucleic Acid Hybridization RNA, Double-Stranded/genetics RNA, Viral/genetics Reoviridae/genetics Rotavirus/genetics
Chemicals
RNA, Double-Stranded RNA, Viral DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Street J E
Croxson M C
Chadderton W F
Bellamy A R
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39 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.43.2.369-378.1982
Published
1982-08-00
Pages
369-78
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256138
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.43.2.369-378.1982 PMC Full Text View journal details
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