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PMID: 6245278 Published · ppublish English Journal Article

Coding assignments of double-stranded RNA segments of SA 11 rotavirus established by in vitro translation.

Journal of virology ·Vol. 33 ·No. 3 ·1980-03-00 ·Pages 976-82

Smith ML, Lazdins I, Holmes IH

Abstract

The segmented double-stranded (ds) RNA genome of the simian rotavirus SA 11, after denaturation, can be translated in a cell-free protein synthesizing system. Of the 11 genome segments, 9 can be resolved on polyacrylamide gels and thus could be individually isolated and translated, providing a means of identifying the polypeptide encoded by each segment. On the basis of electrophoretic mobility of products in sodium dodecyl sulfate-polyacrylamide gels, the probable gene-coding assignments of dsRNA segments 1 to 6 were determined. RNA segments 1 to 4 code for polypeptides I1, I2, I3, and I4, respectively; segment 5 codes for a polypeptide very similar in mobility to a minor polypeptide present in SA 11-infected cells, O1A; and segment 6 codes for the major inner-capsid polypeptide I5.

MeSH Terms
Capsid Genes, Viral Genetic Code Protein Biosynthesis RNA Viruses/genetics RNA, Double-Stranded/genetics RNA, Viral/genetics Rotavirus/genetics,metabolism Viral Proteins/analysis,biosynthesis
Chemicals
RNA, Double-Stranded RNA, Viral Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Smith M L
Lazdins I
Holmes I H
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33 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.33.3.976-982.1980
Published
1980-03-00
Pages
976-82
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC288631
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.33.3.976-982.1980 PMC Full Text View journal details
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