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PMID: 6304692 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Serotype-specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 10 ·1983-05-00 ·Pages 3091-5

Both GW, Mattick JS, Bellamy AR

Abstract

Cloned DNA copies of the double-stranded RNA genomic segments of simian 11 rotavirus have been used to determine the coding assignment for VP7, the type-specific antigen of this virus. Translation of hybrid-selected mRNAs in an in vitro system supplemented with canine pancreatic microsomes permitted VP7 to be assigned to segment 9 and the two nonstructural viral proteins NCVP4 and NCVP3, to segments 7 and 8, respectively. Hybridization of cloned DNA probes for segments 7-9 with the corresponding segments of human rotavirus Wa confirmed these assignments. The complete nucleotide sequence of gene 9 has been determined. The deduced amino acid sequence reveals VP7 to be 326 amino acids in length with two NH2-terminal hydrophobic regions and a single glycosylation site at residues 69-71.

MeSH Terms
Amino Acid Sequence Antigens, Viral/genetics Base Sequence Genes, Viral Glycoproteins/genetics Protein Biosynthesis Rotavirus/genetics Viral Proteins/genetics
Chemicals
Antigens, Viral Glycoproteins Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Both G W
Mattick J S
Bellamy A R
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.10.3091
Published
1983-05-00
Pages
3091-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393980
Subset
IM
Databases
GENBANK
J02354
External Links
PubMed source DOI: 10.1073/pnas.80.10.3091 PMC Full Text View journal details
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