Amikacin resistance mediated by multiresistance transposon Tn2424.

Journal of bacteriology ·Vol. 155 ·No. 2 ·1983-08-00 ·Pages 755-60

Meyer JF, Nies BA, Wiedemann B

Abstract

Tn2424, a multiresistance transposon 25 kilobases long, was isolated from IncFII plasmid NR79. Tn2424 transposed resistance to sulfonamides, streptomycin and spectinomycin, mercuric chloride, chloramphenicol, and amikacin with a frequency of 6 X 10(-5). Resistance to amikacin was mediated by a 6'-N-acetyltransferase, which conferred higher levels of resistance in Pseudomonas aeruginosa than in Escherichia coli. A restriction analysis and cloning experiments resulted in a physical and functional map of Tn2424. Comparison by a heteroduplex technique revealed that Tn2424 includes the total sequence of Tn21 and two additional DNA fragments that are 1.8 and 4 kilobases long.

MeSH Terms

Amikacin/pharmacology Cloning, Molecular DNA Restriction Enzymes DNA Transposable Elements DNA, Bacterial/analysis Drug Resistance, Microbial Escherichia coli/drug effects,genetics Kanamycin/analogs & derivatives Microscopy, Electron Pseudomonas aeruginosa/drug effects,genetics

Chemicals

DNA Transposable Elements DNA, Bacterial Kanamycin Amikacin DNA Restriction Enzymes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Meyer J F

Nies B A

Wiedemann B

References (28)

28 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.155.2.755-760.1983

Published

1983-08-00

Pages

755-60

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC217747

Subset

External Links

PubMed source DOI: 10.1128/jb.155.2.755-760.1983 PMC Full Text View journal details

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Amikacin resistance mediated by multiresistance transposon Tn2424.

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