Home LiteratureArticle Details
PMID: 6279559 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transposition of the carbenicillin-hydrolyzing beta-lactamase gene.

Journal of bacteriology ·Vol. 150 ·No. 2 ·1982-05-00 ·Pages 483-9

Katsu K, Inoue M, Mitsuhashi S

Abstract

We isolated a new transposon Tn2101, from plasmid Rms433 in Enterobacter cloacae. Tn2101 encoded the formation of type IV (carbenicillin-hydrolyzing) beta-lactamase and multiple resistance to streptomycin, sulfanilamide, spectinomycin, and mercury in addition to ampicillin. Tn2101 was transposable between conjugative (or nonconjugative) plasmids and the host chromosome. Transposition occurred independently of the general recombination ability of the host cell. Tn2101 had a molecular size of 9.5 x 10(6) and contained short inverted repeat terminal sequences.

MeSH Terms
Anti-Bacterial Agents/pharmacology Carbenicillin/metabolism Chromosomes, Bacterial DNA Transposable Elements DNA, Bacterial Enterobacter/genetics Enterobacteriaceae/genetics Genes, Bacterial Mercury/pharmacology Molecular Weight Penicillin Resistance Plasmids Recombination, Genetic Repetitive Sequences, Nucleic Acid beta-Lactamases/genetics
Chemicals
Anti-Bacterial Agents DNA Transposable Elements DNA, Bacterial beta-Lactamases Mercury Carbenicillin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Katsu K
Inoue M
Mitsuhashi S
References (25)
25 references, click to expand
  1. Distribution of R factors among Shigella strains isolated in Japan.
    Jpn J Microbiol. 1969 Jun;13(2):187-91 PMID: 5306969
  2. R factor compatibility groups.
    Mol Gen Genet. 1972;119(4):287-97 PMID: 4567804
  3. Molecular specificities of R factor-determined beta-lactamases: correlation with plasmid compatibility.
    J Bacteriol. 1974 Jan;117(1):56-62 PMID: 4587613
  4. A spectrophotometric assay of beta-lactamase action on penicillins.
    Biochem J. 1974 Jun;139(3):789-90 PMID: 4368359
  5. Transposition of ampicillin resistance from RP4 to other replicons.
    Mol Gen Genet. 1974;132(1):31-40 PMID: 4609125
  6. Strand and site specificity of the relaxation event for the relaxation complex of the antibiotic resistance plasmid R6K.
    Biochemistry. 1974 Dec 31;13(27):5484-90 PMID: 4616719
  7. Origin of the TEM-beta-lactamase gene found on plasmids.
    J Bacteriol. 1975 Apr;122(1):250-6 PMID: 1091625
  8. Site specific recA--independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1373-7 PMID: 1093180
  9. Analytical isoelectric focusing of R factor-determined beta-lactamases: correlation with plasmid compatibility.
    J Bacteriol. 1976 Feb;125(2):713-8 PMID: 1107329
  10. EcoRI restriction endonuclease map of the composite R plasmid NR1.
    J Bacteriol. 1976 Jul;127(1):619-36 PMID: 776943
  11. Involvement of multiple translocating DNA segments and recombinational hotspots in the structural evolution of bacterial plasmids.
    J Mol Biol. 1976 Dec;108(2):333-60 PMID: 796463
  12. Transposition of a beta-lactamase locus from RP1 into Pseudomonas putida degradative plasmids.
    J Bacteriol. 1977 Feb;129(2):809-14 PMID: 584205
  13. Gene cloning and containment properties of plasmid Col E1 and its derivatives.
    Science. 1977 Apr 8;196(4286):172-4 PMID: 322277
  14. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  15. Location of an ampicillin resistance transposon, Tn1701, in a group of small, nontransferring plasmids.
    J Bacteriol. 1979 Feb;137(2):990-9 PMID: 370108
  16. Nucleotide sequence of bacteriophage fd DNA.
    Nucleic Acids Res. 1978 Dec;5(12):4495-503 PMID: 745987
  17. R plasmid gene dosage effects in Escherichia coli K-12: copy mutants of the R plasmic R1drd-19.
    Plasmid. 1977 Nov;1(1):1-7 PMID: 375268
  18. Acquisition by Escherichia coli of plasmid-borne beta-lactamases normally confined to Pseudomonas spp.
    Plasmid. 1979 Apr;2(2):269-78 PMID: 377324
  19. Isolation and characterization of a composite plasmid Rms201 mutant temperature sensitive for replication.
    J Bacteriol. 1980 Feb;141(2):577-83 PMID: 6988387
  20. Gene expression of ampicillin resistance transposons, Tn2601 and Tn2602.
    Microbiol Immunol. 1980;24(6):479-94 PMID: 6251343
  21. Transposition of the oxacillin-hydrolyzing penicillinase gene.
    J Bacteriol. 1981 Feb;145(2):808-13 PMID: 6257651
  22. Physical and functional mapping of Tn2603, a transposon encoding ampicillin, streptomycin, sulfonamide, and mercury resistance.
    Mol Gen Genet. 1981;181(4):464-9 PMID: 6267420
  23. Plasmid-mediated carbencillin hydrolyzing beta-lactamases of Proteus mirabilis.
    J Antibiot (Tokyo). 1981 Nov;34(11):1504-5 PMID: 7033194
  24. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  25. Transduction of linked genetic characters of the host by bacteriophage P1.
    Virology. 1955 Jul;1(2):190-206 PMID: 13267987
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.150.2.483-489.1982
Published
1982-05-00
Pages
483-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216392
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.150.2.483-489.1982 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2