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PMID: 4368359 Published · ppublish English Journal Article

A spectrophotometric assay of beta-lactamase action on penicillins.

The Biochemical journal ·Vol. 139 ·No. 3 ·1974-06-00 ·Pages 789-90

Waley SG

Abstract

A new method for measuring the enzymic hydrolysis of the beta-lactam ring in penicillins is described. The change in extinction in the u.v. region is determined. The method is sensitive (50mum-benzylpenicillin can be used) and convenient.

MeSH Terms
Amines Ampicillin Carbenicillin Hydrolysis Penicillanic Acid Penicillin G Penicillinase Penicillins Spectrophotometry, Ultraviolet
Chemicals
Amines Penicillins Ampicillin Penicillanic Acid Penicillinase Carbenicillin Penicillin G
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Waley S G
References (7)
7 references, click to expand
  1. Cephalosporinase and penicillinase activities of a beta-lactamase from Pseudomonas pyocyanea.
    Biochem J. 1965 Sep;96(3):739-52 PMID: 5862414
  2. Computerized microacidimetric determination of beta lactamase Michaelis-Menten constants.
    FEBS Lett. 1973 Jun 15;33(1):42-4 PMID: 4198636
  3. Comparison of assay techniques for beta-lactamase activity.
    Anal Biochem. 1973 Jul;54(1):9-16 PMID: 4725679
  4. A spectrophotometric determination of trypsin and chymotrypsin.
    Biochim Biophys Acta. 1955 Apr;16(4):570-5 PMID: 14389277
  5. A DIRECT SPECTROPHOTOMETRIC ASSAY FOR PENICILLIN BETA-LACTAMASE (PENICILLINASE).
    Biochim Biophys Acta. 1965 Apr 26;99:171-2 PMID: 14325941
  6. 6-Aminopenicillanic acid. V. 6-Aminopenicillanic acid as a substrate for penicillinase and an inducer of penicillinase formation.
    Proc R Soc Lond B Biol Sci. 1961 Aug 15;154:514-21 PMID: 14448514
  7. 6-Aminopenicillanic acid. III. Isolation and purification.
    Proc R Soc Lond B Biol Sci. 1961 Aug 15;154:498-508 PMID: 14448515
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1390789
Published
1974-06-00
Pages
789-90
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1166345
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1390789 PMC Full Text View journal details
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