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PMID: 6304005 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Overproduction of Escherichia coli replication proteins by the use of runaway-replication plasmids.

Journal of bacteriology ·Vol. 154 ·No. 3 ·1983-06-00 ·Pages 1153-61

Yasuda S, Takagi T

Abstract

A derivative of the runaway-replication plasmid was constructed. This plasmid, pSY343, has the gene for kanamycin resistance and single sites for EcoRI, BamHI, HindIII, KpnI, and XhoI that can be used as cloning sites without inactivating the kanamycin resistance gene or the replication genes. Three replication genes of Escherichia coli were cloned on the plasmid. The activity of dnaA, dnaZ, and ssb gene products were 200-, 90-, and 60-fold greater, respectively, in the cells containing these plasmids than in normal cells.

MeSH Terms
Bacterial Proteins/biosynthesis,genetics Carrier Proteins/biosynthesis,genetics Cloning, Molecular DNA Replication Escherichia coli/genetics,metabolism Genes, Bacterial Genetic Vectors Plasmids
Chemicals
Bacterial Proteins Carrier Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yasuda S
Takagi T
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28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.154.3.1153-1161.1983
Published
1983-06-00
Pages
1153-61
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217586
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.154.3.1153-1161.1983 PMC Full Text View journal details
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