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PMID: 368808 Published · ppublish English Journal Article

Bacteriophage lambda carrying the Escherichia coli chromosomal region of the replication origin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 5099-103

Miki T, Hiraga S, Nagata T, Yura T

Abstract

A transducing phage lambdaasn was isolated. The late gene region of its genome was found to have been substituted by an Escherichia coli chromosomal segment containing the genes bgIR, bgIC, glmS, uncA, and asn. Restriction endonuclease cleavage mapping and electron microscopic analysis of the lambdaasn DNA revealed that the size of the bacterial segment is approximately 1.75 X 10(7) daltons, corresponding to about 26.4 kilobases. The circular DNA of lambdaasn was digested with restriction endonuclease EcoRI, diluted, and sealed with DNA ligase. When the reaction mixture was used to transform a recipient E. coli strain, a small plasmid of about 1 X 10(7) daltons (named pMCR115) was obtained. Restriction endonuclease cleavage mapping of pMCR115 and other evidence suggested that it contained the replication origin (oriC) of the E. coli chromosome.

MeSH Terms
Chromosomes, Bacterial Coliphages/genetics DNA Replication DNA, Circular Escherichia coli/genetics Transduction, Genetic Transformation, Bacterial
Chemicals
DNA, Circular
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Miki T
Hiraga S
Nagata T
Yura T
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.10.5099
Published
1978-10-00
Pages
5099-103
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336271
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.10.5099 PMC Full Text View journal details
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