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PMID: 268621 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A DNA fragment containing the origin of replication of the Escherichia coli chromosome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 7 ·1977-07-00 ·Pages 2720-4

Marsh RC, Worcel A

Abstract

A 38 kilobase pair region of the Escherichia coli K12 chromosome containing the replication origin has been physically mapped with restriction endonucleases EcoRI and HindIII. Replication starts within or very near a 1.3 kilobase pair HindIII fragment in the middle of this region and proceeds outward in both directions with apparently equal speed. This pattern was observed in both dnaA and dnaC temperature-sensitive (ts) initiation mutants at the start of the synchronous round of replication which occurs after downshift from the nonpermissive to the permissive temperature.

MeSH Terms
Chromosome Mapping DNA Replication DNA Restriction Enzymes DNA, Bacterial/analysis Molecular Weight Mutation Temperature
Chemicals
DNA, Bacterial DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Marsh R C
Worcel A
References (27)
27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.7.2720
Published
1977-07-00
Pages
2720-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431261
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.7.2720 PMC Full Text View journal details
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