Strains of Escherichia coli diploid for the chromosomal origin of DNA replication.

Molecular & general genetics : MGG ·Vol. 143 ·No. 1 ·1975-12-30 ·Pages 105-11

Masters M

Abstract

F' strains of E. coli have been isolated which are merodiploid for various chromosomal segments between 66 and 78 minutes. Strains diploid for the chromosomal DNA between the genes bgl and mtl grow slowly, have a reduced DNA/mass and an increased cell size. These properties could result if the chromosomal replication origin and a second, extrachromosomal, copy of the origin (located in this case on the F') were to compete for a substance required to initiate replication. We therefore suggest that these strains are diploid for the chromosomal origin of replication and that, therefore, the origin is located between bgl and mtl, that is between 71 and 73 minutes on the E. coli chromosome.

MeSH Terms

Bacterial Proteins/biosynthesis Cell Division Chromosome Mapping Chromosomes, Bacterial Conjugation, Genetic DNA Replication DNA, Bacterial Diploidy Escherichia coli/growth & development,metabolism F Factor Genes Genetic Linkage Models, Biological Mutation Plasmids Transduction, Genetic

Chemicals

Bacterial Proteins DNA, Bacterial

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Masters M

References (16)

16 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00269427

Published

1975-12-30

Pages

105-11

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00269427

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Strains of Escherichia coli diploid for the chromosomal origin of DNA replication.

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