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PMID: 4604144 Published · ppublish English Journal Article

Bacterial cell division regulation: characterization of the dnaH locus of Escherichia coli.

Journal of bacteriology ·Vol. 119 ·No. 2 ·1974-08-00 ·Pages 443-9

Filip CC, Allen JS, Gustafson RA, Allen RG, Walker JR

Abstract

The dnaH locus is the fourth gene to be identified as required for deoxyribonucleic acid polymerization in Escherichia coli. A temperature-sensitive mutant defective in this gene exhibited an abrupt decrease in rate of deoxyribonucleic acid synthesis when shifted to 42 C. The locus mapped in the proC-purE region of the chromosome by conjugation and was co-transducible with purE. dnaH(+) is carried on the F'(13) episome and is dominant over the dnaH(-) mutation.

MeSH Terms
Cell Division Chromosome Mapping Conjugation, Genetic DNA Replication DNA, Bacterial/biosynthesis Deoxyribonucleotides/metabolism Escherichia coli/cytology,metabolism Genes Genes, Dominant Microscopy, Electron Mutation Phosphorus Radioisotopes RNA, Bacterial/biosynthesis Temperature Thymidine/metabolism Transduction, Genetic Tritium Uracil/metabolism
Chemicals
DNA, Bacterial Deoxyribonucleotides Phosphorus Radioisotopes RNA, Bacterial Tritium Uracil Thymidine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Filip C C
Allen J S
Gustafson R A
Allen R G
Walker J R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.119.2.443-449.1974
Published
1974-08-00
Pages
443-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC245626
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.119.2.443-449.1974 PMC Full Text View journal details
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