Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 3150-3

Gefter ML, Hirota Y, Kornberg T, Wechsler JA, Barnoux C

Abstract

A series of double mutants carrying one of the thermosensitive mutations for DNA synthesis (dnaA, B, C, D, E, F, and G) and the polA1 mutation of DeLucia and Cairns, were constructed. Enzyme activities of DNA Polymerases II and III were measured in each mutant. DNA Polymerase II activity was normal in all strains tested. DNA Polymerase III activity is thermosensitive specifically in those strains having thermoscnsitive mutations at the dnaE locus. From these results we conclude that DNA Polymerases II and III are independent enzymes and that DNA Polymerase III is an enzyme required for DNA replication in Escherichia coli.

MeSH Terms

Chromatography DNA Nucleotidyltransferases/analysis,isolation & purification DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/enzymology Hot Temperature Mutation

Chemicals

DNA, Bacterial DNA Nucleotidyltransferases

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Gefter M L

Hirota Y

Kornberg T

Wechsler J A

Barnoux C

References (12)

12 references, click to expand

Isolation of an E. coli strain with a mutation affecting DNA polymerase.
Nature. 1969 Dec 20;224(5225):1164-6 PMID: 4902142
DNA polymerase II.
Nature. 1970 Dec 12;228(5276):1050-3 PMID: 4921664
A new DNA polymerase acitvity of Escherichia coli. II. Properties of the enzyme purified from wild-type E. coli and DNA-ts mutants.
Biochem Biophys Res Commun. 1970 Dec 24;41(6):1565-71 PMID: 4922637
On the process of cellular division in Escherichia coli. 3. Thermosensitive mutants of Escherichia coli altered in the process of DNA initiation.
J Mol Biol. 1970 Nov 14;53(3):369-87 PMID: 4924005
Escherichia coli mutants with temperature-sensitive synthesis of DNA.
Mol Gen Genet. 1970;109(2):107-22 PMID: 4925091
Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division.
Cold Spring Harb Symp Quant Biol. 1968;33:677-93 PMID: 4892005
On the process of cellular division in Escherichia coli. V. Incorporation of deoxynucleoside triphosphates by DNA thermosensitive mutants of Escherichia coli also lacking DNA polymerase activity.
Proc Natl Acad Sci U S A. 1970 Oct;67(2):773-8 PMID: 4943181
Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli.
J Bacteriol. 1951 Sep;62(3):293-300 PMID: 14888646
[Thermosensitive mutants of Escherichia coli K 12. I. Isolation and rapid characterization].
Ann Inst Pasteur (Paris). 1966 Apr;110(4):465-86 PMID: 5325383
DNA transfer and DNA synthesis during bacterial conjugation.
Z Vererbungsl. 1966;98(2):141-9 PMID: 4861724
[Effects of salts on the process of cell division in E. coli].
C R Acad Sci Hebd Seances Acad Sci D. 1969 Mar 3;268(9):1335-8 PMID: 4977057
Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II.
Proc Natl Acad Sci U S A. 1971 Apr;68(4):761-4 PMID: 4927672

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.12.3150

Published

1971-12-00

Pages

3150-3

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389610

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.12.3150 PMC Full Text View journal details

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Analysis of DNA polymerases II and 3 in mutants of Escherichia coli thermosensitive for DNA synthesis.

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