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PMID: 6298730 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA topoisomerases from rat liver: physiological variations.

Nucleic acids research ·Vol. 11 ·No. 4 ·1983-02-25 ·Pages 1059-75

Duguet M, Lavenot C, Harper F, Mirambeau G, De Recondo AM

Abstract

Besides the nicking-closing (topoisomerase I) activity, an ATP-dependent DNA topoisomerase is present in rat liver nuclei. The enzyme, partially purified, is able to catenate in vitro closed DNA circles in a magnesium-dependent, ATP-dependent, histone H1-dependent reaction, and to decatenate in vitro kinetoplast DNA networks to yield free minicircles in a magnesium-dependent and ATP-dependent reaction. It is largely similar to other eukaryotic type II topoisomerases in its requirements, and presumably belongs to this class of enzymes. Type I and type II activities were measured in rat liver nuclei as a function of regenerating time after partial hepatectomy: type I activity was not significantly changed during this process. In contrast, type II activity was considerably increased, suggesting a possible involvement of the enzyme in DNA replication.

MeSH Terms
Animals Cell Nucleus/enzymology DNA Topoisomerases, Type I/isolation & purification,metabolism Isoenzymes/isolation & purification,metabolism Kinetics Liver/enzymology Microscopy, Electron Plasmids Rats
Chemicals
Isoenzymes DNA Topoisomerases, Type I
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Duguet M
Lavenot C
Harper F
Mirambeau G
De Recondo A M
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42 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.4.1059
Published
1983-02-25
Pages
1059-75
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325776
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.4.1059 PMC Full Text View journal details
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