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PMID: 212734 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Antagonists of DNA gyrase inhibit repair and recombination of UV-irradiated phage lambda.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 9 ·1978-09-00 ·Pages 4125-9

Hays JB, Boehmer S

Abstract

Intracellular lambda DNA (from EDTA-sensitive tandem duplication phages) was extracted from infected rec+ bacteria and scored for infectivity and recombination (loss of duplication) by transfection of recA recB spheroplasts and subsequent assay for EDTA resistance. When phage development was blocked by repressor or by antibiotics (chloramphenicol and/or rifampin), the apparent recombination frequency was about 0.1% above the background value for recA infections. Prior irradiation of the phage greatly stimulated recombination; the frequency was 20% when UV fluence was 140 J/m2. Repair (recovery of infectivity) and recombination of irradiated phage DNA proceeded readily in the presence of chloramphenicol and rifampin. Inhibitors of DNA gyrase (coumermycin and oxolinic acid) blocked repair and reduced recombination. UV-stimulated recombination was very low in recA but nearly normal in recB cells: repair was reduced in both mutant strains. The recombination remained high as phage/cell ratios less than unity.

MeSH Terms
Aminocoumarins Anti-Bacterial Agents/pharmacology Coliphages/metabolism Coumarins/pharmacology DNA Repair/drug effects Oxolinic Acid/pharmacology Pyrroles/pharmacology Recombination, Genetic/drug effects,radiation effects Topoisomerase I Inhibitors Ultraviolet Rays
Chemicals
Aminocoumarins Anti-Bacterial Agents Coumarins Pyrroles Topoisomerase I Inhibitors Oxolinic Acid coumermycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hays J B
Boehmer S
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.75.9.4125
Published
1978-09-00
Pages
4125-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336064
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.75.9.4125 PMC Full Text View journal details
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