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PMID: 6298708 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence of the junction in adenovirus 2-SV40 hybrids: examples of illegitimate recombination.

Nucleic acids research ·Vol. 10 ·No. 24 ·1982-12-20 ·Pages 8099-112

Ling LE, Manos MM, Gluzman Y

Abstract

The nucleotide sequences of six Ad2-SV40 junctions from three Ad2-SV40 hybrid viruses (Ad2++HEY, Ad2++LEY and Ad2+D1) were determined. Comparison of parental adenovirus 2 and SV40 DNA sequences with the sequence at the Ad2-SV40 junctions revealed that 5 out of 6 junctions are abrupt transitions from Ad2 to SV40 DNA, and in one case (Ad2++LEY, right junction) there is an additional nucleotide at the junction, which cannot be ascribed to either DNA. Ad2++HEY and Ad2+D1 right junctions are identical and Ad2++LEY and Ad2+ND4 left junctions are identical, a result that strongly suggests these Ad2-SV40 hybrids arose by recombination between the linear Ad2 DNA and circular SV40 DNA, followed by recombination between Ad2 DNA and SV40 DNA present in the Ad2-SV40 hybrid DNA. The unambiguous transition of Ad2 DNA into SV40 DNA at the junction sites is an example of recombination events which have apparently occurred without any homology at the recombination site.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes, Viral Hybridization, Genetic Recombination, Genetic Simian virus 40/genetics
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ling L E
Manos M M
Gluzman Y
References (33)
33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.24.8099
Published
1982-12-20
Pages
8099-112
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327072
Subset
IM
Grants
NCI NIH HHS · CA 13106 · United States
Databases
GENBANK
K01413, K01414, K01415, K01416, K01417, K01418
External Links
PubMed source DOI: 10.1093/nar/10.24.8099 PMC Full Text View journal details
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