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PMID: 6275101 Published · ppublish English Journal Article

Genomic arrangement of an adenovirus-simian virus 40 hybrid virus, Ad2+ND4del.

Journal of virology ·Vol. 40 ·No. 2 ·1981-11-00 ·Pages 526-32

Westphal H

Abstract

Ad2+ND4del is an adenovirus type 2-simian virus 40 hybrid virus nondefective for growth in human cells. The virus was first observed when stocks of Ad2+ND4, a hybrid isolated from primary monkey kidney cells, were propagated in human cells. This paper describes the DNA sequence at two sites of DNA recombination, the site of the left adenovirus type 2-simian virus 40 junction and the site of a deletion of internal simian virus 40 sequences. Since the deletion was observed when the virus was switched from monkey to human cells, an analysis of gene expression in the region of DNA rearrangement may prove useful for the elucidation of molecular events that accompany virus growth in different hosts.

MeSH Terms
Adenoviruses, Human/genetics Base Sequence DNA Restriction Enzymes Deoxyribonuclease HindIII Genes, Viral Recombination, Genetic Simian virus 40/genetics
Chemicals
DNA Restriction Enzymes Deoxyribonuclease HindIII
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Westphal H
References (34)
34 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.40.2.526-532.1981
Published
1981-11-00
Pages
526-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256655
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.40.2.526-532.1981 PMC Full Text View journal details
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