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PMID: 6285300 Published · ppublish English Journal Article

In vitro transcription of two Epstein-Barr virus specified small RNA molecules.

Nucleic acids research ·Vol. 10 ·No. 11 ·1982-06-11 ·Pages 3407-25

Jat P, Arrand JR

Abstract

Cloned DNA from the EcoRI J fragment of EBV has been used as template for in vitro transcription experiments using cell-free extracts prepared from HeLa or KB cells. Two EBV specific RNAs each about 175 bases in length were synthesised and nuclease 51 mapping experiments determined that these in vitro products corresponded precisely to the in vivo species obtained from Raji cells. These two RNA molecules are transcribed by RNA polymerase III and in common with other pol III-synthesised RNAs the coding sequences contain intragenic control regions. The relative abundance of the two RNAs synthesised in vitro differs from that observed in vivo.

MeSH Terms
Base Sequence Burkitt Lymphoma Carcinoma Cell Line Cloning, Molecular DNA Restriction Enzymes Deoxyribonuclease EcoRI HeLa Cells/metabolism Herpesvirus 4, Human/genetics Humans Mouth Neoplasms Nucleic Acid Hybridization Plasmids RNA Polymerase III/metabolism RNA, Viral/genetics Templates, Genetic Transcription, Genetic
Chemicals
RNA, Viral RNA Polymerase III DNA Restriction Enzymes Deoxyribonuclease EcoRI
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jat P
Arrand J R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.11.3407
Published
1982-06-11
Pages
3407-25
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320720
Subset
IM
Databases
GENBANK
J02077
External Links
PubMed source DOI: 10.1093/nar/10.11.3407 PMC Full Text View journal details
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