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PMID: 6940164 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A small nuclear ribonucleoprotein is required for splicing of adenoviral early RNA sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1371-5

Yang VW, Lerner MR, Steitz JA, Flint SJ

Abstract

The size and structure of viral RNA species synthesized in nuclei isolated during the early phase of productive infection by adenovirus type 2 have been examined by electrophoresis in denaturing polyacrylamide cells and the nuclease S1 assay. The major products of transcription in vitro of early regions 1 and 2 in the adenoviral genome are processed RNA molecules that appear to be correctly spliced in isolated nuclei. Splicing of adenoviral RNA molecules is inhibited when nuclei are preincubated with antibodies from systemic lupus erythematosus patients that immunoprecipitate small nuclear ribonucleoprotein particles. The specificity of these antibodies suggests that ribonucleoprotein particles containing U1 RNA are required for splicing of the adenoviral RNA sequences we have examined.

MeSH Terms
Adenoviruses, Human/metabolism Antibodies Cell Nucleus/metabolism HeLa Cells/metabolism Humans Immunoassay Molecular Weight Nucleic Acid Hybridization Nucleoproteins/metabolism RNA, Viral/biosynthesis Ribonucleoproteins/metabolism
Chemicals
Antibodies Nucleoproteins RNA, Viral Ribonucleoproteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yang V W
Lerner M R
Steitz J A
Flint S J
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1371
Published
1981-03-00
Pages
1371-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319132
Subset
IM
Grants
NIGMS NIH HHS · GM26154 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.3.1371 PMC Full Text View journal details
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