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PMID: 232190 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of transcribed regions of Epstein-Barr virus DNA in Burkitt lymphoma-derived cells.

Journal of virology ·Vol. 32 ·No. 1 ·1979-10-00 ·Pages 8-18

Rymo L

Abstract

RNA was extracted from the Burkitt lymphoma-derived cell line Raji and from Burkitt lymphoma tumor biopsies, isotope labeled in vitro by iodination with 125I, and hybridized to electrophoretically separated restriction endonuclease fragments of Epstein-Barr virus DNA on nitrocellulose membranes. The results indicated that only certain parts of the Epstein-Barr virus genome are represented as polyribosomal RNA in Raji cells, with a pronounced dominance of RNA sequences complementary to a 2.0 x 10(6)-dalton segment of Epstein-Barr virus DNA located close to the left end of the viral genome. A map of virus-specific polyribosomal RNA sequences was constructed, which indicated that a minimum of three regions of the Epstein-Barr virus genome are expressed in Raji cells. Total-cell RNA preparations from five Burkitt lymphoma biopsies contained RNA sequences homologous to the same regions of Epstein-Barr virus DNA as polyribosomal RNA from Raji cells, albeit at different relative proportions.

MeSH Terms
Base Sequence Burkitt Lymphoma/analysis Cell Line DNA, Viral/analysis Herpesvirus 4, Human/analysis Humans Nucleic Acid Conformation Nucleic Acid Hybridization RNA, Neoplasm/analysis RNA, Ribosomal/analysis RNA, Viral/analysis
Chemicals
DNA, Viral RNA, Neoplasm RNA, Ribosomal RNA, Viral
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Rymo L
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32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.32.1.8-18.1979
Published
1979-10-00
Pages
8-18
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353521
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.32.1.8-18.1979 PMC Full Text View journal details
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