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PMID: 6774336 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Two control regions for eukaryotic tRNA gene transcription.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 6 ·1980-06-00 ·Pages 3365-8

DeFranco D, Schmidt O, Söll D

Abstract

Two Drosophila tRNALys genes with identical coding sequences were shown to transcribe with very different efficiences in nuclear extracts from Xenopus oocytes. The use of recombinant plasmids in which the 5'-flanking sequences of these genes were either "switched" or replaced by defined pBR322 sequences revealed two control regions for tRNA gene transcription. An internal control region comprising the mature tRNA coding sequence (and possibly its 3'-flanking sequences) is sufficient for transcription initiation, and an external control region comprising the 5'-flanking sequences represses this transcription. All transcripts have short leader sequences. Altered precursor tRNAs transcribed from truncated tRNALys genes (missing a single base pair in the acceptor stem) are not processed well in vitro.

MeSH Terms

Animals Base Sequence Cloning, Molecular DNA, Recombinant/metabolism Drosophila melanogaster/genetics Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics Female Gene Expression Regulation Models, Genetic Oocytes/metabolism Plasmids RNA, Transfer/genetics Templates, Genetic Transcription, Genetic Xenopus/cytology

Chemicals

DNA, Recombinant RNA, Transfer

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

DeFranco D

Schmidt O

Söll D

References (24)

24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.6.3365

Published

1980-06-00

Pages

3365-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC349616

Subset

Databases

GENBANK

J01141, J01151

External Links

PubMed source DOI: 10.1073/pnas.77.6.3365 PMC Full Text View journal details

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Two control regions for eukaryotic tRNA gene transcription.

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