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PMID: 6272200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The conformation of adenovirus VAI-RNA in solution.

Nucleic acids research ·Vol. 9 ·No. 17 ·1981-09-11 ·Pages 4239-50

Monstein HJ, Philipson L

Abstract

The secondary structure of an adenovirus associated low molecular weight RNA (VAI-RNA) has been studied by partial digestion with T1-RNase and S1-endonuclease followed by T1-fingerprint analysis. The empirical secondary structure has been compared with two computer generated models based on minimal free energy of the structure. The results suggest that VAI-RNA in solution has a compact structure with a free energy of around -60 kcal with two stems and four bulge regions. The implication of this structure for the function of VAI-RNA is discussed.

MeSH Terms
Adenoviridae/genetics Base Sequence Computers Electrophoresis, Polyacrylamide Gel Endonucleases/metabolism Humans Hydrolysis Nucleic Acid Conformation RNA, Viral/metabolism Ribonuclease T1/metabolism Single-Strand Specific DNA and RNA Endonucleases
Chemicals
RNA, Viral Endonucleases Ribonuclease T1 Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Monstein H J
Philipson L
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.17.4239
Published
1981-09-11
Pages
4239-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327432
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.17.4239 PMC Full Text View journal details
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