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PMID: 6285289 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure and expression of a Trypanosoma brucei gambiense variant specific antigen gene.

Nucleic acids research ·Vol. 10 ·No. 10 ·1982-05-25 ·Pages 3149-63

Pays E, Lheureux M, Steinert M

Abstract

The expression-linked copy of the T. b. gambiense variant specific antigen gene LiTat 1.6 is transposed in a 20 kb DNA region devoid of restriction sites, located near a chromosome end. This expression site is very similar to that of T. b. brucei variants 117, 118 (1) and AnTat 1.8. In the basic copy, the transposable element (TE) is flanked by repetitive sequences; it includes the gene copy as well as a sequence of 0.9 to 2.1 kb (probably around 1.1 kb) long, upstream from the gene. Probes derived from the 5' part of the TE specifically reveal three polyadenylated transcripts of 4.2, 1.45 and 0.85 kb, respectively, distinct from the 2.1 kb mRNA. The amount of the 4.2 kb sequence is probably less than 0.01% of total trypanosome RNA. Whereas the mRNAs coding for the three isotypic antigens AnTat 1.8 (T. b. brucei), 12.2 (T. b. rhodesiense) and 3.3 (T. evansi) are recognized by LiTat 1.6 probes extending into the 3' half of the transposed sequence, the 5' genomic probes do not hybridize with any of these RNAs. These observations suggest that the LiTat 1.6 gene could be first transcribed in a large precursor molecule. This precursor would be rapidly processed, loosing a large portion of less conserved sequence from its 5' half. Our data are compatible with a model in which the promoter would be provided by the expression site.

MeSH Terms
Animals Antigens/genetics Cloning, Molecular DNA/isolation & purification DNA Restriction Enzymes Genes Genetic Variation Nucleic Acid Hybridization Trypanosoma brucei brucei/genetics
Chemicals
Antigens DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pays E
Lheureux M
Steinert M
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.10.3149
Published
1982-05-25
Pages
3149-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320697
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.10.3149 PMC Full Text View journal details
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