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PMID: 6170573 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isotypic surface glycoproteins of trypanosomes.

Immunology ·Vol. 44 ·No. 2 ·1981-10-00 ·Pages 223-32

Vervoort T, Barbet AF, Musoke AJ, Magnus E, Mpimbaza G, Van Meirvenne N

Abstract

We have identified cloned populations of trypanosomes, derived from different original field isolates and species, which appeared to possess similar variable surface-antigen types when assayed by immune lysis and immunofluorescence tests. Variable surface glycoproteins (VSGs) were isolated from these 'isotypic' trypanosomes. Isolated VSGs had identical apparent mol. wt of 60,000 but different isoelectric points. Radioimmunoassay, using 125I-labelled VSGs and rabbit anti-VSG sera, showed that isotypic VSGs contained not only the cross-reacting determinants found in all other VSGs (Barbet & McGuire, 1978) but also other cross-reacting determinants shared between isotypes. Peptide mapping revealed extensive amino-acid sequence homology between isotype VSGs.

MeSH Terms
Animals Antigens, Surface/analysis,immunology Cross Reactions Electrophoresis, Polyacrylamide Gel Epitopes/analysis Glycoproteins/immunology Peptide Fragments/analysis Radioimmunoassay Species Specificity Trypanosoma/immunology
Chemicals
Antigens, Surface Epitopes Glycoproteins Peptide Fragments
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Vervoort T
Barbet A F
Musoke A J
Magnus E
Mpimbaza G
Van Meirvenne N
References (16)
16 references, click to expand
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1981-10-00
Pages
223-32
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1555224
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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