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PMID: 62018 Published · ppublish English Case Reports Journal Article

Anti-idiotype sera raised against surface immunoglobulin of human neoplastic lymphocytes.

The Journal of experimental medicine ·Vol. 144 ·No. 4 ·1976-10-01 ·Pages 960-9

Hough DW, Eady RP, Hamblin TJ, Stevenson FK, Stevenson GT

Abstract

The idiotypic determinants of surface immunoglobulins on B-cell lymphomas and lymphocytic leukemias represent tumor-specific antigens, individually unique for each tumor. As such they have both diagnostic and therapeutic potential, particularly for those neoplasms with no serum monoclonal immunoglobulin arising from synthesis of the protein for export. We describe the raising in animals of anti-idiotype sera directed against two examples of a nonexporting neoplasm, human chronic lymphocytic leukemia. The procedure involves exposing the cells to papain so as to remove the Fab fragments (containing the idiotypic determinants) from the surface immunoglobulin, recovering the Fab on cellulose immunosorbent particles, and immunizing animals with the immunosorbent-Fab complex.

MeSH Terms
Aged Antibodies, Anti-Idiotypic/biosynthesis Antibody Specificity B-Lymphocytes/immunology Epitopes Female Humans Immunoglobulin Fab Fragments Immunoglobulin M Leukemia, Lymphoid/immunology Male Methods Receptors, Antigen, B-Cell
Chemicals
Antibodies, Anti-Idiotypic Epitopes Immunoglobulin Fab Fragments Immunoglobulin M Receptors, Antigen, B-Cell
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hough D W
Eady R P
Hamblin T J
Stevenson F K
Stevenson G T
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29 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.144.4.960
Published
1976-10-01
Pages
960-9
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190432
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.144.4.960 PMC Full Text View journal details
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