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PMID: 5056670 Published · ppublish English Journal Article

Cell surface immunoglobulin. V. Release from murine splenic lymphocytes.

The Journal of experimental medicine ·Vol. 136 ·No. 4 ·1972-10-01 ·Pages 676-96

Vitetta ES, Uhr JW

Abstract

Turnover and release of cell surface Ig and secretion of total intracellular Ig has been studied in small lymphocytes from normal mouse spleen. The major findings to emerge are: (a) small lymphocytes secrete 8S IgM and IgG. A small portion of the 8S IgM, but virtually none of the IgG appears to have a cell surface phase. (b) Cell surface IgM is actively turned over with a half-life of 6-8 hr, and turnover can be accounted for by release into the incubation medium. Release is temperature dependent. (c) Released cell surface Ig is noncovalently bound to a fragment of plasma membrane. (d) H-2 antigens are not released during short-term incubation. Based on the above findings, we propose a model for the transport and release of both cell surface and conventionally secreted Ig.

MeSH Terms
Animals Cell Membrane/immunology Cells, Cultured Centrifugation, Density Gradient Chloroform/pharmacology Chromatography Cycloheximide/pharmacology Immunoglobulin G/metabolism Immunoglobulins/isolation & purification Iodine Isotopes Lactates Lipid Metabolism Lipoproteins/analysis Lymphocytes/immunology,metabolism Mice Peroxidases/metabolism Spleen/cytology,immunology Splenectomy Tritium Tyrosine/metabolism
Chemicals
Immunoglobulin G Immunoglobulins Iodine Isotopes Lactates Lipoproteins Tritium Tyrosine Chloroform Cycloheximide Peroxidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vitetta E S
Uhr J W
References (36)
36 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.136.4.676
Published
1972-10-01
Pages
676-96
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139271
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.136.4.676 PMC Full Text View journal details
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