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PMID: 5547061 Published · ppublish English Journal Article

Immunoglobulin synthesis and secretion. VI. Synthesis and intracellular transport of immunoglobulin in nonsecretory lymphoma cells.

The Journal of experimental medicine ·Vol. 133 ·No. 4 ·1971-04-01 ·Pages 901-20

Sherr CJ, Uhr JW

Abstract

Cells from an established line of Burkitt's lymphoma (Daudi) and a mouse myeloma (P(3)K) were pulse-labeled in vitro with (3)H-leucine, and immunoglobulin was immunologically precipitated from cell lysates and secretions. In contrast to P(3)K cells, Daudi cells synthesize a small amount of Ig which is not secreted. Subcellular fractionation experiments indicated that Ig of Daudi cells is synthesized on membrane-bound polyribosomes and enters the cisternae of the microsomes. Ig in the microsomes could be labeled with either (3)H-galactose or (3)H-fucose suggesting that transport proceeds to the Golgi complex. Additional evidence indicates that Ig molecules are transported to the plasma membrane but are not cleaved from the cell surface. These results together with other studies of Burkitt lymphoma cells suggest that the Daudi line may represent a clone of neoplastic cells derived from normal lymphocytes which synthesize but do not secrete Ig. Similarities between lymphoma cells and antigen-binding cells are discussed.

MeSH Terms
Biological Transport Burkitt Lymphoma/metabolism Cell Line/metabolism Electrophoresis, Disc Endoplasmic Reticulum/metabolism Fucose/metabolism Galactose/metabolism Immunoglobulin G/metabolism Immunoglobulins/biosynthesis,metabolism Kinetics Leucine/metabolism Microscopy, Electron Microsomes/metabolism Plasma Cells/metabolism Ribosomes/metabolism Tritium
Chemicals
Immunoglobulin G Immunoglobulins Tritium Fucose Leucine Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sherr C J
Uhr J W
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59 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.133.4.901
Published
1971-04-01
Pages
901-20
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138957
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.133.4.901 PMC Full Text View journal details
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