Synthesis of immunoglobulin heavy and light chains by the free ribosomes of a mouse plasma cell tumor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 66 ·No. 2 ·1970-06-00 ·Pages 425-32

Lisowska-Bernstein B, Lamm ME, Vassalli P

Abstract

Free and membrane-bound ribosomes have been isolated from a mouse plasma cell tumor secreting immunoglobulins, and allowed to synthesize proteins under cell-free conditions. Analysis of the products of synthesis by autoradiography of immunoelectrophoresis and peptide maps indicates that both types of ribosomes synthesize heavy and light immunoglobulin chains.

MeSH Terms

Animals Autoradiography Carbon Isotopes Cell Membrane Chromatography, DEAE-Cellulose Chromatography, Gel Immunoelectrophoresis Mice Microscopy, Electron Peptides/analysis Plasmacytoma/metabolism RNA/analysis Ribosomes/drug effects,metabolism gamma-Globulins/biosynthesis

Chemicals

Carbon Isotopes Peptides gamma-Globulins RNA

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Lisowska-Bernstein B

Lamm M E

Vassalli P

References (13)

13 references, click to expand

Preferential synthesis of ferritin and albumin by different populations of liver polysomes.
Science. 1969 May 2;164(3879):584-5 PMID: 5781696
Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver.
J Biol Chem. 1969 Aug 25;244(16):4308-15 PMID: 5806579
Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes.
J Cell Biol. 1970 Apr;45(1):146-57 PMID: 5458993
HERITABLE VARIATION IN BENCE JONES PROTEIN STRUCTURE IN AN INBRED STRAIN OF MICE.
J Mol Biol. 1964 Jun;8:814-22 PMID: 14187407
A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions.
J Biophys Biochem Cytol. 1960 Jul;7:619-30 PMID: 14446510
Free and membrane-bound ribosomes in rat liver.
J Biol Chem. 1967 May 10;242(9):2069-74 PMID: 6022852
Vectorial discharge of peptides released by puromycin from attached ribosomes.
Proc Natl Acad Sci U S A. 1966 Aug;56(2):608-15 PMID: 4961313
Isolation and properties of polyribosomes and fragments of the endoplasmic reticulum from rat liver.
Biochem J. 1967 Apr;103(1):177-82 PMID: 6033756
Ribosomes in rat liver: an estimate of the percentage of free and membrane-bound ribosomes interacting with messenger RNA in vivo.
J Mol Biol. 1967 Sep 28;28(3):539-42 PMID: 6052649
Comparison of guinea pig gamma 1-and gamma 2-immunoglobulins by peptide mapping.
Biochemistry. 1967 Sep;6(9):2819-28 PMID: 4168042
Studies on cell-free synthesis of rat immunoglobulins, I. A cell-free system for protein synthesis prepared from lymph-node microsomal vesicles.
Proc Natl Acad Sci U S A. 1967 Nov;58(5):2117-24 PMID: 4170460
Chemical identification of specific immunoglobulins as the product of a cell-free system from plasmocytoma tumors.
Proc Natl Acad Sci U S A. 1968 Feb;59(2):445-52 PMID: 4171434
Studies on cell-free synthesis of rat immunoglobulins, II. Synthesis of immunoglobulin and of antibody to the dinitrophenyl hapten.
Proc Natl Acad Sci U S A. 1967 Dec;58(6):2422-9 PMID: 4173587

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.66.2.425

Published

1970-06-00

Pages

425-32

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283062

Subset

External Links

PubMed source DOI: 10.1073/pnas.66.2.425 PMC Full Text View journal details

Analysis Services

Synthesis of immunoglobulin heavy and light chains by the free ribosomes of a mouse plasma cell tumor.

Contact

WeChat Official Account