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PMID: 5635036 Published · ppublish English Journal Article

Ultrastructural localization of antibody in differentiating plasma cells.

The Journal of experimental medicine ·Vol. 127 ·No. 1 ·1968-01-01 ·Pages 109-18

Leduc EH, Avrameas S, Bouteille M

Abstract

Antibody was localized by electron microscopy within differentiating and mature plasma cells of the spleens of hyperimmunized rabbits. Horseradish peroxidase was used as antigen. Intracellular antibody to peroxidase was revealed in glutaraldehyde-fixed tissue by coupling it with its antigen and then revealing the sites of peroxidase activity cytochemically. Antibody first appears in the perinuclear space of hemocytoblasts where it persists through differentiation into immature plasma cells, but it disappears from this site in mature plasma cells. Concomitant with the development of the ergastoplasm, antibody accumulates in many but not all of its cisternae. Antibody is present in the lamellar portion of the Golgi apparatus in all phases of plasmacytic differentiation. Mature plasma cells exhibit two types of antibody distribution, a concentration into large spherical intracisternal granules or an overflowing into all parts of the cytoplasm.

MeSH Terms
Animals Antibodies/analysis Antigens Cytoplasm/immunology Cytoplasmic Granules/immunology Golgi Apparatus/immunology Histocytochemistry Microscopy, Electron Peroxidases Plasma Cells/immunology Rabbits Spleen/immunology
Chemicals
Antibodies Antigens Peroxidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Leduc E H
Avrameas S
Bouteille M
References (18)
18 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.127.1.109
Published
1968-01-01
Pages
109-18
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138432
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.127.1.109 PMC Full Text View journal details
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